ArticleFrontiers in physiology2016
Heart Rate and Extracellular Sodium and Potassium Modulation of Gap Junction Mediated Conduction in Guinea Pigs.
Article in Frontiers in physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Electrical Remodeling of Pressure Overloaded Rat Heart Is Attenuated if Imposed During Proliferative Cardiac Growth.Acta physiologica (Oxford, England) · 2025Article
- Sequence-Dependent Repolarization Is Modulated by Endogenous Action Potential Duration Gradients Rather Than Electrical Coupling in Ventricular Myocardium.Journal of the American Heart Association · 2025Article
- Extracellular Perinexal Separation Is a Principal Determinant of Cardiac Conduction.Circulation research · 2023Article
- Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts.Nature communications · 2022Article
- Predictive Capability of Metabolic Panels for Postoperative Atrial Fibrillation in Cardiac Surgery Patients.The Journal of surgical research · 2022Article
- Reevaluating methods reporting practices to improve reproducibility: an analysis of methodological rigor for the Langendorff whole heart technique.American journal of physiology. Heart and circulatory physiology · 2022Review
- Ephaptic Coupling Is a Mechanism of Conduction Reserve During Reduced Gap Junction Coupling.Frontiers in physiology · 2022Article
- Serine-threonine protein phosphatase regulation of Cx43 dephosphorylation in arrhythmogenic disorders.Cellular signalling · 2021Review
- Cellular Size, Gap Junctions, and Sodium Channel Properties Govern Developmental Changes in Cardiac Conduction.Frontiers in physiology · 2021Article
- Elevated perfusate [NaScientific reports · 2020Article
- Attenuating loss of cardiac conduction during no-flow ischemia through changes in perfusate sodium and calcium.American journal of physiology. Heart and circulatory physiology · 2020Article
- Intercellular Sodium Regulates Repolarization in Cardiac Tissue with Sodium Channel Gain of Function.Biophysical journal · 2020Article
- Purification and Application of Genetically Encoded Potassium Ion Indicators for Quantification of Potassium Ion Concentrations within Biological Samples.Current protocols in chemical biology · 2019Article
- The role of the gap junction perinexus in cardiac conduction: Potential as a novel anti-arrhythmic drug target.Progress in biophysics and molecular biology · 2019Review
- The Cardiac Gap Junction has Discrete Functions in Electrotonic and Ephaptic Coupling.Anatomical record (Hoboken, N.J. : 2007) · 2019Article
- Examination of the Effects of Conduction Slowing on the Upstroke of Optically Recorded Action Potentials.Frontiers in physiology · 2019Article
- Quantifying Intermembrane Distances with Serial Image Dilations.Journal of visualized experiments : JoVE · 2018Article
- Intercalated Disk Extracellular Nanodomain Expansion in Patients With Atrial Fibrillation.Frontiers in physiology · 2018Article
- Design and validation of a tissue bath 3-D printed with PLA for optically mapping suspended whole heart preparations.American journal of physiology. Heart and circulatory physiology · 2017Article
- Revealing the Concealed Nature of Long-QT Type 3 Syndrome.Circulation. Arrhythmia and electrophysiology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRecent studies suggested that cardiac conduction in murine hearts with narrow perinexi and 50% reduced connexin43 (Cx43) expression is more sensitive to relatively physiological changes of extracellular potassium ([K(+)]o) and sodium ([Na(+)]o). PURPOSE: Determine whether similar [K(+)]o and [Na(+)]o changes alter conduction velocity (CV) sensitivity to pharmacologic gap junction (GJ) uncoupling in guinea pigs.
methods[K(+)]o and [Na(+)]o were varied in Langendorff perfused guinea pig ventricles (Solution A: [K(+)]o = 4.56 and [Na(+)]o = 153.3 mM. Solution B: [K(+)]o = 6.95 and [Na(+)]o = 145.5 mM). Gap junctions were inhibited with carbenoxolone (CBX) (15 and 30 μM). Epicardial CV was quantified by optical mapping. Perinexal width was measured with transmission electron microscopy. Total and phosphorylated Cx43 were evaluated by western blotting.
resultsSolution composition did not alter CV under control conditions or with 15μM CBX. Decreasing the basic cycle length (BCL) of pacing from 300 to 160 ms decreased CV uniformly with both solutions. At 30 μM CBX, a change in solution did not alter CV either longitudinally or transversely at BCL = 300 ms. However, reducing BCL to 160 ms caused CV to decrease more in hearts perfused with Solution B than A. Solution composition did not alter perinexal width, nor did it change total or phosphorylated serine 368 Cx43 expression. These data suggest that the solution dependent CV changes were independent of altered perinexal width or GJ coupling. Action potential duration was always shorter in hearts perfused with Solution B than A, independent of pacing rate and/or CBX concentration.
conclusionsIncreased heart rate and GJ uncoupling can unmask small CV differences caused by changing [K(+)]o and [Na(+)]o. These data suggest that modulating extracellular ionic composition may be a novel anti-arrhythmic target in diseases with abnormal GJ coupling, particularly when heart rate cannot be controlled.
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