ArticleFrontiers in molecular neuroscience2016
A Toolkit for Orthogonal and in vivo Optical Manipulation of Ionotropic Glutamate Receptors.
Article in Frontiers in molecular neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Optogenetic engineering of synthetic and natural receptors: design principles, functional mechanisms and biomedical applications.Regenerative biomaterials · 2026Review
- Principles and Design of Molecular Tools for Sensing and Perturbing Cell Surface Receptor Activity.Chemical reviews · 2025Review
- Article
- Unveiling the functional diversity of ionotropic glutamate receptors in the Pacific oyster (Frontiers in physiology · 2023Article
- Solid-Phase-Supported Chemoenzymatic Synthesis of a Light-Activatable tRNA Derivative.Angewandte Chemie (International ed. in English) · 2022Article
- Deciphering Molecular Mechanisms and Intervening in Physiological and Pathophysiological Processes of CaCells · 2021Review
- A fine-tuned azobenzene for enhanced photopharmacology in vivo.Cell chemical biology · 2021Article
- Structure, Function, and Pharmacology of Glutamate Receptor Ion Channels.Pharmacological reviews · 2021Review
- Electrophysiology Read-Out Tools for Brain-on-Chip Biotechnology.Micromachines · 2021Review
- Optopharmacology reveals a differential contribution of native GABANeuropharmacology · 2020Article
- Glutamate in Male and Female Sexual Behavior: Receptors, Transporters, and Steroid Independence.Frontiers in behavioral neuroscience · 2020Review
- Optical control of neuronal ion channels and receptors.Nature reviews. Neuroscience · 2019Review
- Holographic two-photon activation for synthetic optogenetics.Nature protocols · 2019Article
- Optical Regulation of Class C GPCRs by Photoswitchable Orthogonal Remotely Tethered Ligands.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Article
- Glutamatergic Signaling in the Central Nervous System: Ionotropic and Metabotropic Receptors in Concert.Neuron · 2018Review
- Light-induced regulation of ligand-gated channel activity.British journal of pharmacology · 2018Review
- Light-Switchable Ion Channels and Receptors for Optogenetic Interrogation of Neuronal Signaling.Bioconjugate chemistry · 2018Review
- Precise modulation of neuronal activity with synthetic photoswitchable ligands.Current opinion in neurobiology · 2017Review
- Article
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Authors and funding
4 authors.
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Abstract
The ability to optically manipulate specific neuronal signaling proteins with genetic precision paves the way for the dissection of their roles in brain function, behavior, and disease. Chemical optogenetic control with photoswitchable tethered ligands (PTLs) enables rapid, reversible and reproducible activation or block of specific neurotransmitter-gated receptors and ion channels in specific cells. In this study, we further engineered and characterized the light-activated GluK2 kainate receptor, LiGluR, to develop a toolbox of LiGluR variants. Low-affinity LiGluRs allow for efficient optical control of GluK2 while removing activation by native glutamate, whereas variant RNA edited versions enable the synaptic role of receptors with high and low Ca(2+) permeability to be assessed and spectral variant photoswitches provide flexibility in illumination. Importantly, we establish that LiGluR works efficiently in the cortex of awake, adult mice using standard optogenetic techniques, thus opening the door to probing the role of specific synaptic receptors and cellular signals in the neural circuit operations of the mammalian brain in normal conditions and in disease. The principals developed in this study are widely relevant to the engineering and in vivo use of optically controllable proteins, including other neurotransmitter receptors.
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