ArticleG3 (Bethesda, Md.)2016
Efficient Genome Editing in Chicken DF-1 Cells Using the CRISPR/Cas9 System.
Article in G3 (Bethesda, Md.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- CRISPR/Cas9-based programmable genome editing in chickens: concepts, applications and regulatory issues.Frontiers in genome editing · 2025Review
- CRISPR/Cas9-mediated knockout of the Vanin-1 gene in the Leghorn Male Hepatoma cell line and its effects on lipid metabolism.Animal bioscience · 2024Article
- Genetically modified chickens as bioreactors for protein-based drugs.Frontiers in genome editing · 2024Review
- Recent Advances in the Application of CRISPR/Cas9 Gene Editing System in Poultry Species.Frontiers in genetics · 2021Review
- Successful CRISPR/Cas9 mediated homologous recombination in a chicken cell line.F1000Research · 2018Article
- Applications of Gene Editing in Chickens: A New Era Is on the Horizon.Frontiers in genetics · 2018Review
- Class 2 CRISPR/Cas: an expanding biotechnology toolbox for and beyond genome editing.Cell & bioscience · 2018Review
- CRISPR/Cas9-Mediated Chicken TBK1 Gene Knockout and Its Essential Role in STING-Mediated IFN-β Induction in Chicken Cells.Frontiers in immunology · 2018Article
- Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo.Developmental biology · 2017Article
- Gene editing in birds takes flight.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- CRISPR/Cas9-mediated p53 and Pten dual mutation accelerates hepatocarcinogenesis in adult hepatitis B virus transgenic mice.Scientific reports · 2017Article
- Potential of gene drives with genome editing to increase genetic gain in livestock breeding programs.Genetics, selection, evolution : GSE · 2017Article
- CRISPR/Cas9 mediated chicken Stra8 gene knockout and inhibition of male germ cell differentiation.PloS one · 2017Article
- Enhancing Targeted Genomic DNA Editing in Chicken Cells Using the CRISPR/Cas9 System.PloS one · 2017Article
- Site-Directed Genome Knockout in Chicken Cell Line and Embryos Can Use CRISPR/Cas Gene Editing Technology.G3 (Bethesda, Md.) · 2016Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recent years, genome engineering technology has provided unprecedented opportunities for site-specific modification of biological genomes. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) 9 is one such means that can target a specific genome locus. It has been applied in human cells and many other organisms. Meanwhile, to efficiently enrich targeted cells, several surrogate systems have also been developed. However, very limited information exists on the application of CRISPR/Cas9 in chickens. In this study, we employed the CRISPR/Cas9 system to induce mutations in the peroxisome proliferator-activated receptor-γ (PPAR-γ), ATP synthase epsilon subunit (ATP5E), and ovalbumin (OVA) genes in chicken DF-1 cells. The results of T7E1 assays showed that the mutation rate at the three different loci was 0.75%, 0.5%, and 3.0%, respectively. In order to improve the mutation efficiency, we used the Puro(R) gene for efficient enrichment of genetically modified cells with the surrogate reporter system. The mutation rate, as assessed via the T7E1 assay, increased to 60.7%, 61.3%, and 47.3%, and subsequent sequence analysis showed that the mutation efficiency increased to 94.7%, 95%, and 95%, respectively. In addition, there were no detectable off-target mutations in three potential off-target sites using the T7E1 assay. As noted above, the CRISPR/Cas9 system is a robust tool for chicken genome editing.
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