ArticleScientific reports2016
Caffeine promotes wakefulness via dopamine signaling in Drosophila.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 85 citations in OpenAlex.
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- RNAi-based screen for pigmentation inbioRxiv : the preprint server for biology · 2025Article
- Nighttime caffeine intake increases motor impulsivity.iScience · 2025Article
- Sleep drive, not total sleep amount, increases seizure risk.Nature communications · 2025Article
- Gut tumors in flies alter the taste valence of an anti-tumorigenic bitter compound.Current biology : CB · 2024Article
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- Downregulation of Plasma Membrane CaFly · 2023Article
- Circadian clock disruption promotes the degeneration of dopaminergic neurons in male Drosophila.Nature communications · 2023Article
- Effects of adenosine receptor overexpression and silencing in neurons and glial cells on lifespan, fitness, and sleep of Drosophila melanogaster.Experimental brain research · 2023Article
- Caffeine Delays Ethanol-Induced Sedation inBiology · 2022Article
- Effect of caffeine on genes expressions of developing retinas in the chick model.Anatomy & cell biology · 2022Article
- Reduced penetrance of Parkinson's disease models.Medizinische Genetik : Mitteilungsblatt des Berufsverbandes Medizinische Genetik e.V · 2022Article
- Dopaminergic Ric GTPase activity impacts amphetamine sensitivity and sleep quality in a dopamine transporter-dependent manner in Drosophila melanogaster.Molecular psychiatry · 2021Article
- Compartment specific regulation of sleep by mushroom body requires GABA and dopaminergic signaling.Scientific reports · 2021Article
- Acute effect of cola and caffeine on locomotor activity in drosophila and rat.Physiological research · 2021Article
- Fermented Gamma Aminobutyric Acid Improves Sleep Behaviors in Fruit Flies and Rodent Models.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Caffeine is the most widely-consumed psychoactive drug in the world, but our understanding of how caffeine affects our brains is relatively incomplete. Most studies focus on effects of caffeine on adenosine receptors, but there is evidence for other, more complex mechanisms. In the fruit fly Drosophila melanogaster, which shows a robust diurnal pattern of sleep/wake activity, caffeine reduces nighttime sleep behavior independently of the one known adenosine receptor. Here, we show that dopamine is required for the wake-promoting effect of caffeine in the fly, and that caffeine likely acts presynaptically to increase dopamine signaling. We identify a cluster of neurons, the paired anterior medial (PAM) cluster of dopaminergic neurons, as the ones relevant for the caffeine response. PAM neurons show increased activity following caffeine administration, and promote wake when activated. Also, inhibition of these neurons abrogates sleep suppression by caffeine. While previous studies have focused on adenosine-receptor mediated mechanisms for caffeine action, we have identified a role for dopaminergic neurons in the arousal-promoting effect of caffeine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.