ArticleJournal of ovarian research2016
Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival.
Article in Journal of ovarian research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Technological Advances of Cryopreservation in Ovarian Tissue for Female Children: Exploring the Molecular Insights and Mechanisms.International journal of molecular sciences · 2026Review
- Treatment with trypLE before freezing improves thawing integrity and functionality of sheep ovarian tissue.Journal of ovarian research · 2025Article
- Roles of follicle stimulating hormone and sphingosine 1-phosphate co-administered in the process in mouse ovarian vitrification and transplantation.Journal of ovarian research · 2023Article
- The mTOR Inhibitor Rapamycin Counteracts Follicle Activation Induced by Ovarian Cryopreservation in Murine Transplantation Models.Medicina (Kaunas, Lithuania) · 2023Article
- Iron overload triggering ECM-mediated Hippo/YAP pathway in follicle development: a hypothetical model endowed with therapeutic implications.Frontiers in endocrinology · 2023Review
- Pharmacological inhibition of the PI3K/PTEN/Akt and mTOR signalling pathways limits follicle activation induced by ovarian cryopreservation and in vitro culture.Journal of ovarian research · 2021Article
- Applying plastic surgery principles to ovarian tissue transplantation.Gland surgery · 2021Review
- In Vitro Activation Early Follicles: From the Basic Science to the Clinical Perspectives.International journal of molecular sciences · 2021Review
- Ovarian Fragmentation and AKT Stimulation for Expansion of Fertile Lifespan.Frontiers in reproductive health · 2021Review
- Impact of S1P Mimetics on Mesenteric Ischemia/Reperfusion Injury.Pharmaceuticals (Basel, Switzerland) · 2020Article
- Evaluation of Z-VAD-FMK as an anti-apoptotic drug to prevent granulosa cell apoptosis and follicular death after human ovarian tissue transplantation.Journal of assisted reproduction and genetics · 2019Article
- Structure of preantral follicles, oxidative status and developmental competence of in vitro matured oocytes after ovary storage at 4 °C in the domestic cat model.Reproductive biology and endocrinology : RB&E · 2018Article
- A transportation network for human ovarian tissue is indispensable to success for fertility preservation.Journal of assisted reproduction and genetics · 2017Review
- Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model.Journal of assisted reproduction and genetics · 2016Article
- Ovarian tissue transport to expand access to fertility preservation: from animals to clinical practice.Reproduction (Cambridge, England) · 2016Review
- Is Ovarian Tissue Transport at Supra-zero Temperatures Compared to Body Temperature Optimal for Follicle Survival?In vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOvarian tissue preservation is proposed to patients at risk of premature ovarian failure, but this procedure still needs to be optimized. To limit injury during ovarian tissue cryopreservation, anti-apoptotic drugs were added to the transport and freezing media of ovarian cortex tissue.
methodsSheep ovaries were transported, prepared and frozen in solutions containing vehicle or anti-apoptotic drugs (Z-VAD-FMK, a pan-caspase inhibitor, or sphingosine-1-phosphate (S1P), a bioactive lipid). After the tissue was thawed, the ovarian cortex was cultured for 2 or 6 days. Follicular quantification and morphological and proliferation analyses were performed on histological sections.
resultsAfter 2 days of culture, S1P improved the quality of primordial follicles; higher densities of morphologically normal and proliferative primordial follicles were found. Z-VAD-FMK displayed similar effects by preserving global primordial follicular density, but this effect was evident after 6 days of culture. This drug also improved cell proliferation after 2 and 6 days of culture.
conclusionsOur results showed that the addition of S1P or Z-VAD-FMK to the transport and freezing media prior to ovarian tissue cryopreservation improves primordial follicular quality and therefore improves global tissue survival. This should ultimately lead to improved fertility restoration after auto-transplantation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.