ArticleBehavioral and brain functions : BBF2016
Non-targeted metabolite profiling reveals changes in oxidative stress, tryptophan and lipid metabolisms in fearful dogs.
Article in Behavioral and brain functions : BBF, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 40 citations in OpenAlex.
- Investigating targeted and untargeted approaches to biomarker discovery in fearful, adult dogs.Journal of animal science · 2026Article
- Article
- Metabolomics provides novel understanding of Melissa officinalis mechanism of action ensuring its calming effect on dogs.BMC veterinary research · 2025Article
- Resveratrol Ameliorates Chronic Stress in Kennel Dogs and Mice by Regulating Gut Microbiome and Metabolome Related to Tryptophan Metabolism.Antioxidants (Basel, Switzerland) · 2025Article
- Metabolic profiles show few differences in serum amino acid, one-carbon, and fatty acid compounds in dogs fed a plant-based ("vegan") or meat-based diet.Frontiers in veterinary science · 2025Article
- Treats containing cannabidiol, L-tryptophan andFrontiers in veterinary science · 2025Article
- Metabolomics Reveal Key Metabolic Pathway Responses to Anxiety State Regulated by Serotonin inMetabolites · 2024Article
- Postprandial Plasma and Whole Blood Amino Acids Are Largely Indicative of Dietary Amino Acids in Adult Dogs Consuming Diets with Increasing Whole Pulse Ingredient Inclusion.The Journal of nutrition · 2024Article
- New Advances in Attention-Deficit/Hyperactivity Disorder-like Dogs.Animals : an open access journal from MDPI · 2024Review
- Integrating (Nutri-)Metabolomics into the One Health Tendency-The Key for Personalized Medicine Advancement.Metabolites · 2023Review
- Urinary neurotransmitter analysis and canine behavior assessment.Frontiers in veterinary science · 2023Article
- The Future of Biomarkers in Veterinary Medicine: Emerging Approaches and Associated Challenges.Animals : an open access journal from MDPI · 2022Review
- Plasma metabolomic profiles associated with chronic distress in women.Psychoneuroendocrinology · 2021Article
- Anxiety disturbs the blood plasma metabolome in acute coronary syndrome patients.Scientific reports · 2021Article
- Metabolomics in the study of spontaneous animal diseases.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2020Review
- Metabolome of canine and human saliva: a non-targeted metabolomics study.Metabolomics : Official journal of the Metabolomic Society · 2020Article
- Active and social life is associated with lower non-social fearfulness in pet dogs.Scientific reports · 2020Article
- A novel genomic region on chromosome 11 associated with fearfulness in dogs.Translational psychiatry · 2020Article
- Canine metabolomics advances.Metabolomics : Official journal of the Metabolomic Society · 2020Review
- Genome-wide association studies of 74 plasma metabolites of German shepherd dogs reveal two metabolites associated with genes encoding their enzymes.Metabolomics : Official journal of the Metabolomic Society · 2019Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnxieties, such as shyness, noise phobia and separation anxiety, are common but poorly understood behavioural problems in domestic dogs, Canis familiaris. Although studies have demonstrated genetic and environmental contributions to anxiety pathogenesis, better understanding of the molecular underpinnings is needed to improve diagnostics, management and treatment plans. As a part of our ongoing canine anxiety genetics efforts, this study aimed to pilot a metabolomics approach in fearful and non-fearful dogs to identify candidate biomarkers for more objective phenotyping purposes and to refer to potential underlying biological problem.
methodsWe collected whole blood samples from 10 fearful and 10 non-fearful Great Danes and performed a liquid chromatography combined with mass spectrometry (LC-MS)-based non-targeted metabolite profiling.
resultsNon-targeted metabolomics analysis detected six 932 metabolite entities in four analytical modes [RP and HILIC; ESI(-) and ESI(+)], of which 239 differed statistically between the test groups. We identified changes in 13 metabolites (fold change ranging from 1.28 to 2.85) between fearful and non-fearful dogs, including hypoxanthine, indoxylsulfate and several phospholipids. These molecules are involved in oxidative stress, tryptophan and lipid metabolisms.
conclusionsWe identified significant alterations in the metabolism of fearful dogs, and some of these changes appear relevant to anxiety also in other species. This pilot study demonstrates the feasibility of the non-targeted metabolomics and warrants a larger replication study to confirm the role of the identified biomarkers and pathways in canine anxiety.
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