Evidence map›Paper›PMID 26867772›Full record

ArticleTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine2016

Exosomal miRNAs as biomarkers of recurrent lung cancer.

Radha Munagala, Farrukh Aqil, Ramesh C Gupta

Abstract read
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In one paragraph

Article in Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
66citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

66 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  5. Article
  6. Relationship between exosomes and cancer: formation, diagnosis, and treatment.International journal of biological sciences · 2025
    Review
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  8. Exosomal microRNAs in lung cancer: a narrative review.Translational cancer research · 2024
    Review
  9. Article
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  16. Article
  17. Tumor-derived exosomal non-coding RNAs as diagnostic biomarkers in cancer.Cellular and molecular life sciences : CMLS · 2022
    Review
  18. Review
  19. Review
  20. The role of Exosomal miRNAs in cancer.Journal of translational medicine · 2022
    Review

6 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Radha MunagalaDepartment of Medicine, University of Louisville, 580 S. Preston Street, Louisville, KY, 40202, USA. r0muna01@louisville.edu.
Farrukh AqilDepartment of Medicine, University of Louisville, 580 S. Preston Street, Louisville, KY, 40202, USA.
Ramesh C GuptaJames Graham Brown Cancer Center, University of Louisville, 580 S. Preston Street, Louisville, KY, 40202, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prognosis of lung cancer still remains grim largely due to recurrence and aggressive metastasis of the disease. In this study, we examined the potential of exosomal miRNAs as biomarkers of recurrent lung cancer. Initially, in vitro miRNA profiles of normal lung (Beas-2b) and lung cancer (H1299) cells and of exosomes isolated from conditioned media were determined. In vivo study involved establishing subcutaneous primary and recurrent lung cancer xenografts in nude mouse model and examining tumor and serum exosomal miRNA alteration in secondary/recurrent lung tumors. A total of 77 miRNAs were observed to be significantly modulated in the H1299 cells (47 miRNA upregulated and 30 downregulated) compared to the Beas-2b cells. The exosomes isolated from conditioned media indicated several miRNAs which were in agreement with cells of origin. A similarity was also observed between miRNAs from serum exosomes and tumors, indicating their origin from the lung tumors. Two miRNAs, miR-21 and miR-155, were found to be significantly upregulated in recurrent tumors compared to primary tumors. These miRNAs were also upregulated in serum exosomes of recurrent tumor-bearing animals versus non-tumor- or primary tumor-bearing animals. Increased expression of the recurrent disease markers were also observed in recurrent tumors compared with primary tumors. Serum exosomes from recurrent tumor mice mirrored its tumor profile in expressing higher levels of these proteins compared with exosomes from primary tumor mice. Our data suggest that exosomal miRNA signatures may be a true representation of a pathological profile of lung cancer; thus, miRNAs could serve as promising biomarkers for non-invasive diagnosis of the disease.

Indexed as

AnimalsBiomarkers, TumorCell Line, TumorCulture Media, ConditionedEpithelial CellsExosomesHeterograftsHumansLung NeoplasmsMiceMice, NudeMicroRNAsNeoplasm ProteinsRecurrenceRNA, NeoplasmBiomarkers, TumorCulture Media, ConditionedMicroRNAsNeoplasm ProteinsRNA, NeoplasmBiomarkerExosomesLung cancermiRNARecurrent lung tumors

Identifiers

PMID26867772

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.