ArticleScientific reports2016
Characterization of SCO4439, a D-alanyl-D-alanine carboxypeptidase involved in spore cell wall maturation, resistance, and germination in Streptomyces coelicolor.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 31 citations in OpenAlex.
- Peptidoglycan-reshuffling proteins SCO0954, SCO1758, SCO4439, and SCO4440 modulate the formation of wall-deficient cells in Streptomyces coelicolor under hyperosmotic sucrose stress.Scientific reports · 2025Article
- Genome Sequences of the First Phages InfectingMicroorganisms · 2025Article
- The DeoR-like pleiotropic regulator SCO1897 controls specialised metabolism, sporulation, spore germination, and phosphorus accumulation in Streptomyces coelicolor.Communications biology · 2024Article
- Lactic acid bacteria secreted proteins as potential Listeria monocytogenes quorum sensing inhibitors.Molecular diversity · 2024Article
- Identification of a putative cell wall-hydrolyzing amidase involved in sporangiospore maturation inJournal of bacteriology · 2024Article
- PredictingMicroorganisms · 2024Article
- FtsZ phosphorylation pleiotropically affects Z-ladder formation, antibiotic production, and morphogenesis in Streptomyces coelicolor.Antonie van Leeuwenhoek · 2023Article
- Penicillin-Binding Proteins, β-Lactamases, and β-Lactamase Inhibitors in β-Lactam-Producing Actinobacteria: Self-Resistance Mechanisms.International journal of molecular sciences · 2022Review
- The SCO2102 Protein Harbouring a DnaA II Protein-Interaction Domain Is Essential for the SCO2103 Methylenetetrahydrofolate Reductase Positioning atInternational journal of molecular sciences · 2022Article
- A Systematic Immuno-Informatic Approach to Design a Multiepitope-Based Vaccine Against Emerging Multiple Drug ResistantFrontiers in immunology · 2022Article
- Genomic Insights into the Distribution and Phylogeny of Glycopeptide Resistance Determinants within theAntibiotics (Basel, Switzerland) · 2021Article
- The Modulation ofInternational journal of molecular sciences · 2021Article
- Subtractive proteomics approach to Unravel the druggable proteins of the emerging pathogenHeliyon · 2021Article
- Structural, Metabolic and Evolutionary Comparison of Bacterial Endospore and Exospore Formation.Frontiers in microbiology · 2021Review
- Identification and Characterization of a Cell Wall Hydrolase for Sporangiospore Maturation in Actinoplanes missouriensis.Journal of bacteriology · 2019Article
- Cytosolic copper is a major modulator of germination, development and secondary metabolism in Streptomyces coelicolor.Scientific reports · 2019Article
- High-Resolution Analysis of the Peptidoglycan Composition in Streptomyces coelicolor.Journal of bacteriology · 2018Article
- Quantitative Proteome and Phosphoproteome Analyses ofMolecular & cellular proteomics : MCP · 2018Article
- Streptomyces Differentiation in Liquid Cultures as a Trigger of Secondary Metabolism.Antibiotics (Basel, Switzerland) · 2018Review
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
This work contributes to the understanding of cell wall modifications during sporulation and germination in Streptomyces by assessing the biological function and biochemical properties of SCO4439, a D-alanyl-D-alanine carboxypeptidase (DD-CPase) constitutively expressed during development. SCO4439 harbors a DD-CPase domain and a putative transcriptional regulator domain, separated by a putative transmembrane region. The recombinant protein shows that DD-CPase activity is inhibited by penicillin G. The spores of the SCO4439::Tn5062 mutant are affected in their resistance to heat and acid and showed a dramatic increase in swelling during germination. The mycelium of the SCO4439::Tn5062 mutant is more sensitive to glycopeptide antibiotics (vancomycin and teicoplanin). The DD-CPase domain and the hydrophobic transmembrane region are highly conserved in Streptomyces, and both are essential for complementing the wild type phenotypes in the mutant. A model for the biological mechanism behind the observed phenotypes is proposed, in which SCO4439 DD-CPase releases D-Ala from peptidoglycan (PG) precursors, thereby reducing the substrate pool for PG crosslinking (transpeptidation). PG crosslinking regulates spore physical resistance and germination, and modulates mycelium resistance to glycopeptides. This study is the first demonstration of the role of a DD-CPase in the maturation of the spore cell wall.
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