ArticleThe Journal of biological chemistry2016
Control of the Position of Oxygen Delivery in Soybean Lipoxygenase-1 by Amino Acid Side Chains within a Gas Migration Channel.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Activation and execution of lipoxygenase catalysis.Redox biochemistry and chemistry · 2026Article
- Bacterial lipoxygenases for fatty acid oxyfunctionalization: advances and future directions.Applied microbiology and biotechnology · 2026Review
- The presence of substrate warrants oxygen access tunnels toward the catalytic site of lipoxygenases.Redox biology · 2025Article
- Elucidating the Role of OACS environmental Au · 2024Article
- Directed evolution unlocks oxygen reactivity for a nicotine-degrading flavoenzyme.Nature chemical biology · 2023Article
- Soybean (International journal of molecular sciences · 2023Article
- Programming xenon diffusion in maltose-binding protein.Biophysical journal · 2022Article
- Structure of lactate oxidase from Enterococcus hirae revealed new aspects of active site loop function: Product-inhibition mechanism and oxygen gatekeeper.Protein science : a publication of the Protein Society · 2022Article
- Review
- Small-Molecule Tunnels in Metalloenzymes Viewed as Extensions of the Active Site.Accounts of chemical research · 2021Review
- Fatty Acid Allosteric Regulation of C-H Activation in Plant and Animal Lipoxygenases.Molecules (Basel, Switzerland) · 2020Review
- EPR Spectroscopic Studies of Lipoxygenases.Chemistry, an Asian journal · 2020Review
- The Biosynthesis of Enzymatically Oxidized Lipids.Frontiers in endocrinology · 2020Review
- Moving Through Barriers in Science and Life.Annual review of biochemistry · 2019Article
- Impact of Mutations on the Binding Pocket of Soybean Lipoxygenase: Implications for Proton-Coupled Electron Transfer.The journal of physical chemistry letters · 2018Article
- Fast Hydrogen Atom Abstraction by a Hydroxo Iron(III) Porphyrazine.Journal of the American Chemical Society · 2017Article
- Article
- Synthesis of Site-SpecificallyTetrahedron letters · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Understanding gas migration pathways is critical to unraveling structure-function relationships in enzymes that process gaseous substrates such as O2, H2, and N2 This work investigates the role of a defined pathway for O2 in regulating the peroxidation of linoleic acid by soybean lipoxygenase 1. Computational and mutagenesis studies provide strong support for a dominant delivery channel that shuttles molecular oxygen to a specific region of the active site, thereby ensuring the regio- and stereospecificity of product. Analysis of reaction kinetics and product distribution in channel mutants also reveals a plasticity to the gas migration pathway. The findings show that a single site mutation (I553W) limits oxygen accessibility to the active site, greatly increasing the fraction of substrate that reacts with oxygen free in solution. They also show how a neighboring site mutation (L496W) can result in a redirection of oxygen toward an alternate position of the substrate, changing the regio- and stereospecificity of peroxidation. The present data indicate that modest changes in a protein scaffold may modulate the access of small gaseous molecules to enzyme-bound substrates.
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