Evidence map›Paper›PMID 26867574›Full record

ArticleThe Journal of biological chemistry2016

Ras-related C3 Botulinum Toxin Substrate (Rac) and Src Family Kinases (SFK) Are Proximal and Essential for Phosphatidylinositol 3-Kinase (PI3K) Activation in Natural Killer (NK) Cell-mediated Direct Cytotoxicity against Cryptococcus neoformans.

Richard F Xiang, Danuta Stack, Shaunna M Huston, Shu Shun Li, Henry Ogbomo, Stephen K Kyei, Christopher H Mody

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.9field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 17 citations in OpenAlex.

  1. Review
  2. Article
  3. Immune Cell Degranulation in Fungal Host Defence.Journal of fungi (Basel, Switzerland) · 2021
    Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. The study of transcriptome sequencing in childhood immune thrombocytopenia.Blood coagulation & fibrinolysis : an international journal in haemostasis and thrombosis · 2019
    Article
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Richard F XiangFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
Danuta StackFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
Shaunna M HustonFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
Shu Shun LiFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
Henry OgbomoFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
Stephen K KyeiFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada.
Christopher H ModyFrom the Departments of Microbiology, Immunology and Infectious Diseases and the Snyder Institute for Chronic Disease, University of Calgary, Calgary, Alberta T2N 4N1, Canada Internal Medicine and cmody@ucalgary.ca.
University of Calgary · CA

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

The activity of Rac in leukocytes is essential for immunity. However, its role in NK cell-mediated anti-microbial signaling remains unclear. In this study, we investigated the role of Rac in NK cell mediated anti-cryptococcal killing. We found thatCryptococcus neoformansindependently activates both Rac and SFK pathways in NK cells, and unlike in tumor killing,Cryptococcusinitiated a novel Rac → PI3K → Erk cytotoxicity cascade. Remarkably, Rac was not required for conjugate formation, despite its essential role in NK cytotoxicity againstC. neoformans Taken together, our data show that, unlike observations with tumor cells, NK cells use a novel Rac cytotoxicity pathway in conjunction with SFK, to killC. neoformans.

Indexed as

Cytotoxicity, ImmunologicCell Line, TumorClass Ia Phosphatidylinositol 3-KinaseCryptococcus neoformansGene Expression RegulationHost-Pathogen InteractionsHumansKiller Cells, NaturalMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3PhosphorylationPrimary Cell CulturePyronesQuinolinesrac1 GTP-Binding ProteinRAC2 GTP-Binding ProteinClass Ia Phosphatidylinositol 3-KinaseEHT 1864MAPK1 protein, humanMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3PyronesQuinolinesrac1 GTP-Binding ProteinRAC1 protein, humanRAC2 GTP-Binding Proteinrac GTP-Binding ProteinsRNA, Small Interferingsrc-Family KinasesadhesionCellular signalingCryptococcusfunginatural killer cells (NK cells)phosphatidylinositide 3-kinase (PI 3-kinase)Rac (Rac GTPase)Src

Identifiers

PMID26867574
PMCPMC4807276
OpenAlexW2281522039

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.