Evidence map›Paper›PMID 26867493›Full record

ArticleJournal of hepatology2016

The pro-inflammatory effects of miR-155 promote liver fibrosis and alcohol-induced steatohepatitis.

Shashi Bala, Timea Csak, Banishree Saha, James Zatsiorsky, Karen Kodys, Donna Catalano, Abhishek Satishchandran, Gyongyi Szabo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of hepatology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 178 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
178citing papers in PubMed, 2 pooled it
9.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

178 citing papers in PubMed, 2 syntheses or guidelines pooled it, 297 citations in OpenAlex.

  1. Pooled it
  2. Role of miR-155 in inflammatory autoimmune diseases: a comprehensive review.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2022
    Pooled it
  3. A Resistance Training Program on Patients with Liver Cirrhosis: A Randomized Clinical Trial.International journal of environmental research and public health · 2025
    Trial
  4. Article
  5. Review
  6. Article
  7. Rutin attenuates liver fibrosis via the IRG1-itaconate-Nrf2 axis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
    Article
  8. Cuproptosis: potential new direction in liver-related diseases research and treatment.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026
    Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Review
  14. Review
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article

118 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Shashi BalaDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA.
Timea CsakDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA; Brookdale University Hospital and Medical Center, Brooklyn, NY, USA.
Banishree SahaDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA.
James ZatsiorskyDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA.
Karen KodysDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA.
Donna CatalanoDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA.
Abhishek SatishchandranDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA.
Gyongyi SzaboDepartment of Medicine, University of Massachusetts Medical School, Worcester 01604, MA, USA. Electronic address: gyongyi.szabo@umassmed.edu.
University of Massachusetts Chan Medical School · US

Funding

MicroRNAs in Alcoholic Liver Disease Administrative SupplementR01AA020744 · NIAAA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SZABO, GYONGYI · 2011 to 2023
$5.3M
Medical Scientist Training at UMMS Administrative SupplementT32GM107000 · NIGMS · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI KIEFE, CATARINA I. · 2013 to 2022
$4.9M
Bio distribution and function of alcohol-induced exRNAR21AA023628 · NIAAA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI SZABO, GYONGYI · 2015 to 2016
$431k
Mass spectrometric investigation of biomolecules and druZ01DK070004 · NIDDK · DIABETES, DIGESTIVE, KIDNEY DISEASES · PI HESS, SONJA · 2005 to 2006
–
Intramural NIH HHS Z01 DK070004NIAAA NIH HHS R01 AA020744NIAAA NIH HHS R21 AA023628NIGMS NIH HHS T32 GM107000NLM NIH HHS HHSN267200700004C
6 · The paper itself

Abstract

BACKGROUND &

aimsAlcoholic liver disease (ALD) ranges from fatty liver to inflammation and cirrhosis. miRNA-155 is an important regulator of inflammation. In this study, we describe the in vivo role of miR-155 in ALD.

methodsWild-type (WT) (C57/BL6J) or miR-155 knockout (KO) and TLR4 KO mice received Lieber DeCarli diet for 5weeks. Some mice received corn oil or CCl4 for 2 or 9weeks.

resultsWe found that miR-155 KO mice are protected from alcohol-induced steatosis and inflammation. The reduction in alcohol-induced fat accumulation in miR-155 KO mice was associated with increased peroxisome proliferator-activated receptor response element (PPRE) and peroxisome proliferator-activated receptors (PPAR)α (miR-155 target) binding and decreased MCP1 production. Treatment with a miR-155 inhibitor increased PPARγ expression in naïve and alcohol treated RAW macrophages. Alcohol increased lipid metabolism gene expression (FABP4, LXRα, ACC1 and LDLR) in WT mice and this was prevented in KO mice. Alcohol diet caused an increase in the number of CD163(+) CD206(+) infiltrating macrophages and neutrophils in WT mice, which was prevented in miR-155 KO mice. Kupffer cells isolated from miR-155 KO mice exhibited predominance of M2 phenotype when exposed to M1 polarized signals and this was due to increased C/EBPβ. Pro-fibrotic genes were attenuated in miR-155 KO mice after alcohol diet or CCl4 treatment. Compared to WT mice, attenuation in CCl4 induced hydroxyproline and α-SMA was observed in KO mice. Finally, we show TLR4 signaling regulates miR-155 as TLR4 KO mice showed no induction of miR-155 after alcohol diet.

conclusionsCollectively our results demonstrated the role of miR-155 in alcohol-induced steatohepatitis and fibrosis in vivo.

Indexed as

AnimalsCarbon TetrachlorideDNAFatty Liver, AlcoholicFemaleInterferon-gammaLiver Cirrhosis, ExperimentalMiceMice, Inbred C57BLMice, KnockoutMicroRNAsPPAR alphaResponse ElementsToll-Like Receptor 4Carbon TetrachlorideDNAInterferon-gammaMicroRNAsMirn155 microRNA, mousePPAR alphaTlr4 protein, mouseToll-Like Receptor 4AlcoholFibrosisInflammationmicroRNAPPARαPPARγ

Identifiers

PMID26867493
PMCPMC4874886
OpenAlexW2261068749

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.