ArticleNature protocols2016
Procedures for the reconstruction, primary culture and experimental use of rainbow trout gill epithelia.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Voltage-gated ion channels in cultured gill epithelia of rainbow trout, Oncorhynchus mykiss, change in transcript abundance with exposure to freshwater.Comparative biochemistry and physiology. Part A, Molecular & integrative physiology · 2025Article
- Making in vitro conditions more reflective of in vivo conditions for research on the teleost gastrointestinal tract.The Journal of experimental biology · 2024Review
- Review
- In Vivo Functional Assay in Fish Gills: Exploring Branchial Acid-Excreting Mechanisms in Zebrafish.International journal of molecular sciences · 2022Article
- Sea Bass Primary Cultures versus RTgill-W1 Cell Line: Influence of Cell Model on the Sensitivity to Nanoparticles.Nanomaterials (Basel, Switzerland) · 2021Article
- Establishment of a striped catfish skin explant model for studying the skin response in Aeromonas hydrophila infections.Scientific reports · 2021Article
- Cultured rainbow trout gill epithelium as anHeliyon · 2021Article
- Molecular and biochemical characterization of the bicarbonate-sensing soluble adenylyl cyclase from a bony fish, the rainbow troutInterface focus · 2021Article
- Co-exposure to polystyrene plastic beads and polycyclic aromatic hydrocarbon contaminants in fish gill (RTgill-W1) and intestinal (RTgutGC) epithelial cells derived from rainbow trout (Oncorhynchus mykiss).Environmental pollution (Barking, Essex : 1987) · 2019Article
- The Use of Molecular Descriptors To Model Pharmaceutical Uptake by a Fish Primary Gill Cell Culture Epithelium.Environmental science & technology · 2019Article
- Establishment and long-term maintenance of primary intestinal epithelial cells cultured from the rainbow trout,Biology open · 2018Article
- A fish intestinal epithelial barrier model established from the rainbow trout (Oncorhynchus mykiss) cell line, RTgutGC.Cell biology and toxicology · 2017Article
- Investigations to extend viability of a rainbow trout primary gill cell culture.Ecotoxicology (London, England) · 2017Article
- Application of the rainbow trout derived intestinal cell line (RTgutGC) for ecotoxicological studies: molecular and cellular responses following exposure to copper.Ecotoxicology (London, England) · 2017Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
This protocol describes how to reconstruct and culture the freshwater rainbow trout gill epithelium on flat permeable membrane supports within cell culture inserts. The protocol describes gill cell isolation, cultured gill epithelium formation, maintenance, monitoring and preparation for use in experimental procedures. To produce a heterogeneous gill epithelium, as seen in vivo, seeding of isolated gill cells twice over a 2-d period is required. As a consequence, this is termed the double-seeded insert technique. Approximately 5-12 d after cell isolation and seeding, preparations develop electrically tight gill epithelia that can withstand freshwater on the apical cell surface. The system can be used to study freshwater gill physiology, and it is a humane alternative for toxicity testing, bioaccumulation studies and environmental water quality monitoring.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.