ArticleJournal of medical imaging (Bellingham, Wash.)2016
Active monitoring of formaldehyde diffusion into histological tissues with digital acoustic interferometry.
Article in Journal of medical imaging (Bellingham, Wash.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Perfusion fixation in brain banking: a systematic review.Acta neuropathologica communications · 2019Pooled it
- Clinical advances in analytical profiling of signature lipids: implications for severe non-communicable and neurodegenerative diseases.Metabolomics : Official journal of the Metabolomic Society · 2024Review
- Mapping the primate thalamus: systematic approach to analyze the distribution of subcortical neuromodulatory afferents.Brain structure & function · 2023Article
- Assessing Tissue Fixation Time and Quality with Label-free Mid Infrared Spectroscopy and Machine Learning.Biopreservation and biobanking · 2023Article
- The BrainboxBrain communications · 2023Article
- Human Brain Lipidomics: Investigation of Formalin Fixed Brains.Frontiers in molecular neuroscience · 2022Article
- Making a science out of preanalytics: An analytical method to determine optimal tissue fixation in real-time.PloS one · 2021Article
- Monitoring Dehydration and Clearing in Tissue Processing for High-Quality Clinical Pathology.Biopreservation and biobanking · 2019Article
- Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues.Emerging infectious diseases · 2019Article
- Evaluation of Formalin Fixation for Tissue Biopsies Using Shear Wave Laser Speckle Imaging System.IEEE journal of translational engineering in health and medicine · 2019Article
- A New Paradigm for Tissue Diagnostics: Tools and Techniques to Standardize Tissue Collection, Transport, and Fixation.Current pathobiology reports · 2018Review
- Precision Medicine Starts With Preanalytics: Real-Time Assessment of Tissue Fixation Quality by Ultrasound Time-of-Flight Analysis.Applied immunohistochemistry & molecular morphology : AIMM · 2017Article
- Impaired coordination between signaling pathways is revealed in human colorectal cancer using single-cell mass cytometry of archival tissue blocks.Science signaling · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The preservation of certain labile cancer biomarkers with formaldehyde-based fixatives can be considerably affected by preanalytical factors such as quality of fixation. Currently, there are no technologies capable of quantifying a fixative's concentration or the formation of cross-links in tissue specimens. This work examined the ability to detect formalin diffusion into a histological specimen in real time. As formaldehyde passively diffused into tissue, an ultrasound time-of-flight (TOF) shift of several nanoseconds was generated due to the distinct sound velocities of formalin and exchangeable fluid within the tissue. This signal was resolved with a developed digital acoustic interferometry algorithm, which compared the phase differential between signals and computed the absolute TOF with subnanosecond precision. The TOF was measured repeatedly across the tissue sample for several hours until diffusive equilibrium was realized. The change in TOF from 6-mm thick ex vivo human tonsil fit a single-exponential decay ([Formula: see text]) with rate constants that varied drastically spatially between 2 and 10 h ([Formula: see text]) due to substantial heterogeneity. This technology may prove essential to personalized cancer diagnostics by documenting and tracking biospecimen preanalytical fixation, guaranteeing their suitability for diagnostic assays, and speeding the workflow in clinical histopathology laboratories.
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