ArticleBiomicrofluidics2016
A microfluidic electrochemical biosensor based on multiwall carbon nanotube/ferrocene for genomic DNA detection of Mycobacterium tuberculosis in clinical isolates.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Innovative Carbonaceous Materials and Metal/Metal Oxide Nanoparticles for Electrochemical Biosensor Applications.Nanomaterials (Basel, Switzerland) · 2024Review
- Recent Developments in Aptamer-Based Sensors for Diagnostics.Sensors (Basel, Switzerland) · 2024Review
- Pre-enrichment-free detection of hepatocellular carcinoma-specific ctDNA via PDMS and MEMS-based microfluidic sensor.Mikrochimica acta · 2024Article
- Aptamer-based rapid diagnosis for point-of-care application.Microfluidics and nanofluidics · 2023Review
- Sensors Based on the Carbon Nanotube Field-Effect Transistors for Chemical and Biological Analyses.Biosensors · 2022Review
- Microfluidics as a Novel Technique for Tuberculosis: From Diagnostics to Drug Discovery.Microorganisms · 2021Review
- Carbon Nanotube Field-Effect Transistor-Based Chemical and Biological Sensors.Sensors (Basel, Switzerland) · 2021Review
- Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2021Review
- Graphene Oxide Based Electrochemical Genosensor for Label Free Detection ofInternational journal of nanomedicine · 2021Article
- HCV Detection, Discrimination, and Genotyping Technologies.Sensors (Basel, Switzerland) · 2018Review
- Carbon-Based Nanomaterials for Biomedical Applications: A Recent Study.Frontiers in pharmacology · 2018Review
- Lab-on-chip technology for chronic disease diagnosis.NPJ digital medicine · 2018Review
- Carbon nanotubes in microfluidic lab-on-a-chip technology: current trends and future perspectives.Microfluidics and nanofluidics · 2017Article
- Gene Specific DNA Sensors for Diagnosis of Pathogenic Infections.Indian journal of microbiology · 2017Review
- Label-Free and Regenerative Electrochemical Microfluidic Biosensors for Continual Monitoring of Cell Secretomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2017Article
- Electrochemical Genosensing of Circulating Biomarkers.Sensors (Basel, Switzerland) · 2017Review
- Diagnostics Strategies with Electrochemical Affinity Biosensors Using Carbon Nanomaterials as Electrode Modifiers.Diagnostics (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Herein we present a microfluidic-multiplexed platform that integrates electrochemical sensors based on carbon nanotubes associated with ferrocene as redox marker (carbon nanotube (CNT)/ferrocene) for direct detection of pathogenic viral DNA from Hepatitis C and genomic DNA from Mycobacterium tuberculosis in clinical isolates. By operating the fluidic device under high flow (150 μl/min), the formation of a very thin depletion layer at the sensor surface (δS = 230 nm) enhances the capture rate up to one DNA strand per second. By comparison, this capture rate is only 0.02 molecule/s in a static regime without flow. This fluidic protocol allows thus enhancing the limit of detection of the electrochemical biosensor from picomolar in bulk solution to femtomolar with a large dynamic range from 0.1 fM to 1 pM. Kinetics analysis also demonstrates an enhancement of the rate constant of electron transfer (kS) of the electrochemical process from 1 s(-1) up to 6 s(-1) thanks to the geometry of the miniaturized fluidic electrochemical cell. This microfluidic device working under high flow allows selective direct detection of a Mycobacterium tuberculosis (H37Rv) rpoB allele from clinical isolate extracted DNA. We envision that a microfluidic approach under high flow associated with a multiwall CNT/ferrocene sensor could find useful applications as the point-of-care for multi-target diagnostics of biomarkers in real samples.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.