Evidence map›Paper›PMID 26865686›Full record

ArticleJournal of clinical microbiology2016

Comparative Detection and Quantification of Arcobacter butzleri in Stools from Diarrheic and Nondiarrheic People in Southwestern Alberta, Canada.

Andrew L Webb, Valerie F Boras, Peter Kruczkiewicz, L Brent Selinger, Eduardo N Taboada, G Douglas Inglis

Open access · bronzeAbstract read
In one paragraph

Article in Journal of clinical microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
7.4field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.

  1. Pooled it
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  13. Genomic Characterization ofFrontiers in microbiology · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Andrew L WebbAgriculture and Agri-Food Canada, Lethbridge, AB, Canada Department of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.
Valerie F BorasDepartment of Laboratory Medicine, Chinook Regional Hospital, Lethbridge, AB, Canada.
Peter KruczkiewiczPublic Health Agency of Canada, Lethbridge, AB, Canada.
L Brent SelingerDepartment of Biological Sciences, University of Lethbridge, Lethbridge, AB, Canada.
Eduardo N TaboadaPublic Health Agency of Canada, Lethbridge, AB, Canada douglas.inglis@agr.gc.ca eduardo.taboada@phac-aspc.gc.ca.
G Douglas InglisAgriculture and Agri-Food Canada, Lethbridge, AB, Canada douglas.inglis@agr.gc.ca eduardo.taboada@phac-aspc.gc.ca.
Agriculture and Agri-Food Canada · CAPublic Health Agency of Canada · CAChinook Regional Hospital · CAUniversity of Lethbridge · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Arcobacter butzleri has been linked to enteric disease in humans, but its pathogenicity and epidemiology remain poorly understood. The lack of suitable detection methods is a major limitation. Using comparative genome analysis, we developed PCR primers for direct detection and quantification ofA. butzleri DNA in microbiologically complex matrices. These primers, along with existing molecular and culture-based methods, were used to detectA. butzleri and enteric pathogens in stools of diarrheic and nondiarrheic people (n= 1,596) living in southwestern Alberta, Canada, from May to November 2008. In addition, quantitative PCR was used to compare A. butzleridensities in diarrheic and nondiarrheic stools.Arcobacter butzleriwas detected more often by PCR (59.6%) than by isolation methods (0.8%). Comparison by PCR-based detection found no difference in the prevalence ofA. butzleri between diarrheic (56.7%) and nondiarrheic (45.5%) individuals. Rates of detection in diarrheic stools peaked in June (71.1%) and October (68.7%), but there was no statistically significant correlation between the presence ofA. butzleri and patient age, sex, or place of habitation. Densities ofA. butzleriDNA in diarrheic stools (1.6 ± 0.59 log10 copies mg(-1)) were higher (P= 0.007) than in nondiarrheic stools (1.3 ± 0.63 log10copies mg(-1)). Of the 892 diarrheic samples that were positive for A. butzleri, 74.1% were not positive for other bacterial and/or viral pathogens. The current study supports previous work suggesting that A. butzleri pathogenicity is strain specific and/or dependent on other factors, such as the level of host resistance.

Indexed as

AdolescentAdultAgedAged, 80 and overAlbertaArcobacterBacterial LoadChildChild, PreschoolDiarrheaFecesFemaleGram-Negative Bacterial InfectionsHumansInfantInfant, Newborn

Identifiers

PMID26865686
PMCPMC4809925
OpenAlexW2260764139

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.