ArticleThe Journal of biological chemistry2016
Hexokinase 2 Is an Intracellular Glucose Sensor of Yeast Cells That Maintains the Structure and Activity of Mig1 Protein Repressor Complex.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
51 citing papers in PubMed, 91 citations in OpenAlex.
- Glucose repression ofbioRxiv : the preprint server for biology · 2026Article
- Transcriptional Reprogramming of Lifespan-Associated Genes in Yeast Lacking TOR1.Biochemical genetics · 2026Article
- Review
- Multilayered control of hexose uptake and phosphorylation through specialized sugar kinases in Nakaseomyces glabratus.Fungal biology and biotechnology · 2026Article
- The small GTPase Rho5-Yet another player in yeast glucose signaling.PLoS genetics · 2025Article
- Possible regulatory network and associated pathways governing the expression of ADH2 in Saccharomyces cerevisiae.Current genetics · 2025Review
- Advances and trends for astaxanthin synthesis in Phaffia rhodozyma.Microbial cell factories · 2025Review
- Sugar-sensing swodkoreceptors and swodkocrine signaling.Animal models and experimental medicine · 2025Article
- The Yeast Gsk-3 Kinase Mck1 Is Necessary for Cell Wall Remodeling in Glucose-Starved and Cell Wall-Stressed Cells.International journal of molecular sciences · 2025Article
- OsFKBP12 transduces the sucrose signal from OsNIN8 to the OsTOR pathway in a loosely binding manner for cell division.iScience · 2025Article
- Evaluation of Stress Tolerance and Fermentation Performance in Commercial Yeast Strains for Industrial Applications.Foods (Basel, Switzerland) · 2025Article
- The role of hexokinases in epigenetic regulation: altered hexokinase expression and chromatin stability in yeast.Epigenetics & chromatin · 2024Article
- Glycolytic enzymes in non-glycolytic web: functional analysis of the key players.Cell biochemistry and biophysics · 2024Review
- Coupling High-Throughput and Targeted Screening for Identification of Nonobvious Metabolic Engineering Targets.ACS synthetic biology · 2024Article
- Article
- Exploring carbon source related localization and phosphorylation in the Snf1/Mig1 network using population and single cell-based approaches.Microbial cell (Graz, Austria) · 2024Article
- Glucose metabolism in the pathogenic free-living amoebae: Tempting targets for treatment development.Chemical biology & drug design · 2024Review
- Regulation of Fungal Morphogenesis and Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2023Article
- Quantifying intracellular glucose levels when yeast is grown in glucose media.Scientific reports · 2023Article
- Changing course: Glucose starvation drives nuclear accumulation of Hexokinase 2 in S. cerevisiae.PLoS genetics · 2023Article
Corrections and comments
- Commented on by
- Commented on by
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hexokinase 2 (Hxk2) fromSaccharomyces cerevisiaeis a bi-functional enzyme, being both a catalyst in the cytosol and an important regulator of the glucose repression signal in the nucleus. Despite considerable recent progress, little is known about the regulatory mechanism that controls nuclear Hxk2 association with theSUC2promoter chromatin and how this association is necessary forSUC2gene repression. Our data indicate that in theSUC2promoter context, Hxk2 functions through a variety of structurally unrelated factors, mainly the DNA-binding Mig1 and Mig2 repressors and the regulatory Snf1 and Reg1 factors. Hxk2 sustains the repressor complex architecture maintaining transcriptional repression at theSUC2gene. Using chromatin immunoprecipitation assays, we discovered that the Hxk2 in its open configuration, at low glucose conditions, leaves the repressor complex that induces its dissociation and promotesSUC2gene expression. In high glucose conditions, Hxk2 adopts a close conformation that promotes Hxk2 binding to the Mig1 protein and the reassembly of theSUC2repressor complex. Additional findings highlight the possibility that Hxk2 constitutes an intracellular glucose sensor that operates by changing its conformation in response to cytoplasmic glucose levels that regulate its interaction with Mig1 and thus its recruitment to the repressor complex of theSUC2promoter. Thus, our data indicate that Hxk2 is more intimately involved in gene regulation than previously thought.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.