ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2016
QRFP and Its Receptors Regulate Locomotor Activity and Sleep in Zebrafish.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 56 citations in OpenAlex.
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- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.Current biology : CB · 2026Article
- Neuroprotective effects of traditional Chinese medicine using zebrafish models - a review.Frontiers in pharmacology · 2026Review
- Pth4 neurons define a novel hypothalamic circuit that promotes sleep via brainstem monoaminergic neurons.bioRxiv : the preprint server for biology · 2025Article
- Body temperature regulates glucose metabolism and torpid behavior.Nature communications · 2025Article
- The neuropeptide FLP-11 induces and self-inhibits sleep through the receptor DMSR-1 in Caenorhabiditis elegans.Current biology : CB · 2025Article
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- Structural basis for recognition of 26RFa by the pyroglutamylated RFamide peptide receptor.Cell discovery · 2024Article
- Genetic ablation of the Bsx homeodomain transcription factor in zebrafish: Impact on mature pineal gland morphology and circadian behavior.Journal of pineal research · 2022Article
- The evolutionarily conserved miRNA-137 targets the neuropeptide hypocretin/orexin and modulates the wake to sleep ratio.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Zebrafish as a Tool in the Study of Sleep and Memory-related Disorders.Current neuropharmacology · 2022Review
- Validation of Candidate Sleep Disorder Risk Genes Using Zebrafish.Frontiers in molecular neuroscience · 2022Review
- Recent advances in sleep genetics.Current opinion in neurobiology · 2021Review
- The variations in human orphan G protein-coupled receptor QRFPR affect PI3K-AKT-mTOR signaling.Journal of clinical laboratory analysis · 2021Article
- Elevated preoptic brain activity in zebrafish glial glycine transporter mutants is linked to lethargy-like behaviors and delayed emergence from anesthesia.Scientific reports · 2021Article
- Neuromodulation and Behavioral Flexibility in Larval Zebrafish: From Neurotransmitters to Circuits.Frontiers in molecular neuroscience · 2021Article
- Heterogeneity of Hypocretin/Orexin Neurons.Frontiers of neurology and neuroscience · 2021Review
- Review
- Sleep Circuits and Physiology in Non-Mammalian Systems.Current opinion in physiology · 2020Article
- Molecular codes and in vitro generation of hypocretin and melanin concentrating hormone neurons.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The hypothalamus plays an important role in regulating sleep, but few hypothalamic sleep-promoting signaling pathways have been identified. Here we demonstrate a role for the neuropeptide QRFP (also known as P518 and 26RFa) and its receptors in regulating sleep in zebrafish, a diurnal vertebrate. We show that QRFP is expressed in ∼10 hypothalamic neurons in zebrafish larvae, which project to the hypothalamus, hindbrain, and spinal cord, including regions that express the two zebrafish QRFP receptor paralogs. We find that the overexpression of QRFP inhibits locomotor activity during the day, whereas mutation of qrfp or its receptors results in increased locomotor activity and decreased sleep during the day. Despite the restriction of these phenotypes to the day, the circadian clock does not regulate qrfp expression, and entrained circadian rhythms are not required for QRFP-induced rest. Instead, we find that QRFP overexpression decreases locomotor activity largely in a light-specific manner. Our results suggest that QRFP signaling plays an important role in promoting sleep and may underlie some aspects of hypothalamic sleep control. SIGNIFICANCE STATEMENT: The hypothalamus is thought to play a key role in regulating sleep in vertebrate animals, but few sleep-promoting signaling pathways that function in the hypothalamus have been identified. Here we use the zebrafish, a diurnal vertebrate, to functionally and anatomically characterize the neuropeptide QRFP. We show that QRFP is exclusively expressed in a small number of neurons in the larval zebrafish hypothalamus that project widely in the brain. We also show that QRFP overexpression reduces locomotor activity, whereas animals that lack QRFP signaling are more active and sleep less. These results suggest that QRFP signaling participates in the hypothalamic regulation of sleep.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.