ArticleAnnals of botany2016
Pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method.
Article in Annals of botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Low Temperature Suppress Cell Wall Degradation by Modulating Antioxidant Metabolism in Figs.Foods (Basel, Switzerland) · 2026Article
- Cold storage delays peach fruit softening via mMolecular horticulture · 2026Article
- Understanding Chilling Injury and Sugar Metabolism-Related Genes and Metabolites in 'Red Haven' Peaches.Plants (Basel, Switzerland) · 2025Article
- Determining the function of ripening associated genes and biochemical changes during tomato (Solanum lycopersicum L.) fruit maturation.Biotechnology letters · 2025Article
- Fruit softening: evidence for rhamnogalacturonan lyase action in vivo in ripe fruit cell walls.Annals of botany · 2024Article
- Expansin SlExp1 and endoglucanase SlCel2 synergistically promote fruit softening and cell wall disassembly in tomato.The Plant cell · 2024Article
- How Extracellular Reactive Oxygen Species Reach Their Intracellular Targets in Plants.Molecules and cells · 2023Review
- ROS generated from biotic stress: Effects on plants and alleviation by endophytic microbes.Frontiers in plant science · 2022Review
- Article
- Cuticle and skin cell walls have common and unique roles in grape berry splitting.Horticulture research · 2021Article
- Cutin:cutin-acid endo-transacylase (CCT), a cuticle-remodelling enzyme activity in the plant epidermis.The Biochemical journal · 2021Article
- Identification of DNA Methylation and Transcriptomic Profiles Associated With Fruit Mealiness inFrontiers in plant science · 2021Article
- Two Carbohydrate-Based Natural Extracts StimulateFrontiers in plant science · 2021Article
- Genome wide identification and functional characterization of strawberry pectin methylesterases related to fruit softening.BMC plant biology · 2020Article
- Characterization of CRISPR Mutants Targeting Genes Modulating Pectin Degradation in Ripening Tomato.Plant physiology · 2019Article
- Genome re-sequencing reveals the evolutionary history of peach fruit edibility.Nature communications · 2018Article
- The oxidation of dehydroascorbic acid and 2,3-diketogulonate by distinct reactive oxygen species.The Biochemical journal · 2018Article
- Congruence between PM HProtoplasma · 2018Article
- Sugar metabolism reprogramming in a non-climacteric bud mutant of a climacteric plum fruit during development on the tree.Journal of experimental botany · 2017Article
- Novel insights into ascorbate retention and degradation during the washing and post-harvest storage of spinach and other salad leaves.Food chemistry · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND AND
aimsMany fruits soften during ripening, which is important commercially and in rendering the fruit attractive to seed-dispersing animals. Cell-wall polysaccharide hydrolases may contribute to softening, but sometimes appear to be absent. An alternative hypothesis is that hydroxyl radicals ((•)OH) non-enzymically cleave wall polysaccharides. We evaluated this hypothesis by using a new fluorescent labelling procedure to 'fingerprint' (•)OH-attacked polysaccharides.
methodsWe tagged fruit polysaccharides with 2-(isopropylamino)-acridone (pAMAC) groups to detect (a) any mid-chain glycosulose residues formed in vivo during (•)OH action and (b) the conventional reducing termini. The pAMAC-labelled pectins were digested with Driselase, and the products resolved by high-voltage electrophoresis and high-pressure liquid chromatography. KEY
resultsStrawberry, pear, mango, banana, apple, avocado, Arbutus unedo, plum and nectarine pectins all yielded several pAMAC-labelled products. GalA-pAMAC (monomeric galacturonate, labelled with pAMAC at carbon-1) was produced in all species, usually increasing during fruit softening. The six true fruits also gave pAMAC·UA-GalA disaccharides (where pAMAC·UA is an unspecified uronate, labelled at a position other than carbon-1), with yields increasing during softening. Among false fruits, apple and strawberry gave little pAMAC·UA-GalA; pear produced it transiently.
conclusionsGalA-pAMAC arises from pectic reducing termini, formed by any of three proposed chain-cleaving agents ((•)OH, endopolygalacturonase and pectate lyase), any of which could cause its ripening-related increase. In contrast, pAMAC·UA-GalA conjugates are diagnostic of mid-chain oxidation of pectins by (•)OH. The evidence shows that (•)OH radicals do indeed attack fruit cell wall polysaccharides non-enzymically during softening in vivo. This applies much more prominently to drupes and berries (true fruits) than to false fruits (swollen receptacles). (•)OH radical attack on polysaccharides is thus predominantly a feature of ovary-wall tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.