Evidence map›Paper›PMID 26865378›Full record

ArticleMolecular imaging and biology2016

Noninvasive Monitoring of the Mitochondrial Function in Mesenchymal Stromal Cells.

Federico Franchi, Karen M Peterson, Ramasamy Paulmurugan, Clifford Folmes, Ian R Lanza, Amir Lerman, Martin Rodriguez-Porcel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular imaging and biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. NoninvasiveWorld journal of stem cells · 2020
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Federico FranchiDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.
Karen M PetersonDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.
Ramasamy PaulmuruganDepartment of Radiology and Molecular Imaging Program at Stanford (MIPS), Stanford University School of Medicine, Stanford, CA, USA.
Clifford FolmesDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.
Ian R LanzaDivision of Endocrinology and Metabolism, Department of Internal Medicine, Mayo Clinic, Rochester, MN, USA.
Amir LermanDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.
Martin Rodriguez-PorcelDivision of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA. rodriguez.m@mayo.edu.
WinnMed · USMayo Clinic · USMayo Clinic in Arizona · USStanford University · US

Funding

Imaging mitochondrial function of progenitor cells transplanted to the ischemic myocardiumR01HL119795 · NHLBI · MAYO CLINIC ROCHESTER · PI RODRIGUEZ-PORCEL, MARTIN · 2016 to 2020
$3.1M
Molecular Sensors for Imaging Histone Methylations in Living AnimalsR01CA161091 · NCI · STANFORD UNIVERSITY · PI PAULMURUGAN, RAMASAMY · 2012 to 2015
$1.3M
Non-invasive imaging of progenitor cell fate in the ischemic myocardiumR56HL113371 · NHLBI · MAYO CLINIC ROCHESTER · PI RODRIGUEZ-PORCEL, MARTIN · 2013 to 2013
$533k
Metabolic reprogramming in pluripotent induction and cardiac lineage specificatioK99HL121079 · NHLBI · MAYO CLINIC ROCHESTER · PI FOLMES, CLIFFORD D · 2014 to 2015
$279k
NCI NIH HHS R01 CA161091NHLBI NIH HHS K99 HL121079NHLBI NIH HHS R01 HL119795NHLBI NIH HHS R56 HL113371
6 · The paper itself

Abstract

purposeMitochondria are a gatekeeper of cell survival and mitochondrial function can be used to monitor cell stress. Here we validate a pathway-specific reporter gene to noninvasively image the mitochondrial function of stem cells. PROCEDURES: We constructed a mitochondrial sensor with the firefly luciferase (Fluc) reporter gene driven by the NQO1 enzyme promoter. The sensor was introduced in stem cells and validated in vitro and in vivo, in a mouse model of myocardial ischemia/reperfusion (IR).

resultsThe sensor activity showed an inverse relationship with mitochondrial function (R (2) = -0.975, p = 0.025) and showed specificity and sensitivity for mitochondrial dysfunction. In vivo, NQO1-Fluc activity was significantly higher in IR animals vs. controls, indicative of mitochondrial dysfunction, and was corroborated by ex vivo luminometry.

conclusionsReporter gene imaging allows assessment of the biology of transplanted mesenchymal stromal cells (MSCs), providing important information that can be used to improve the phenotype and survival of transplanted stem cells.

Indexed as

AnimalsBiomarkersCell TrackingGenes, ReporterGenetic VectorsMaleatesMesenchymal Stem CellsMiceMitochondriaReproducibility of ResultsBiomarkersMaleatesmaleic acidBioluminescenceMesenchymal stem cellsMitochondrial functionReporter gene

Identifiers

PMID26865378
PMCPMC4966544
OpenAlexW2271724562

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.