ArticleScientific reports2016
An integrated platform for large-scale data collection and precise perturbation of live Drosophila embryos.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed.
- A phenomics approach reveals interspecific differences in integrated developmental responses to chronic elevated temperatures.The Journal of experimental biology · 2023Article
- Comparative phenomics: a new approach to study heterochrony.Frontiers in physiology · 2023Article
- Phenomics as an approach to Comparative Developmental Physiology.Frontiers in physiology · 2023Article
- Exploiting the Potential ofBiomedicines · 2021Review
- Microfluidics on the fly: Inexpensive rapid fabrication of thermally laminated microfluidic devices for live imaging and multimodal perturbations of multicellular systems.Biomicrofluidics · 2019Article
- Tools to reverse-engineer multicellular systems: case studies using the fruit fly.Journal of biological engineering · 2019Review
- Parallel imaging ofDisease models & mechanisms · 2017Article
- Three-dimensional models for studying development and disease: moving on from organisms to organs-on-a-chip and organoids.Integrative biology : quantitative biosciences from nano to macro · 2016Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Understanding the fundamental principles governing embryogenesis is a key goal of developmental biology. Direct observation of embryogenesis via in vivo live imaging is vital to understanding embryogenesis; yet, tedious sample preparation makes it difficult to acquire large-scale imaging data that is often required to overcome experimental and biological noises for quantitative studies. Furthermore, it is often difficult, and sometimes impossible, to incorporate environmental perturbation for understanding developmental responses to external stimuli. To address this issue, we have developed a method for high-throughput imaging of live embryos, delivering precise environmental perturbations, and unbiased data extraction. This platform includes an optimized microfluidic device specifically for live embryos and also for precise perturbations in the microenvironment of the developing embryos. In addition, we developed software for simple, yet accurate, automated segmentation of fluorescent images, and automated data extraction. Using a quantitative assessment we find that embryos develop normally within the microfluidic device. Finally, we show an application of the high-throughput assay for monitoring developmental responses to external stimuli: anoxia-induced developmental arrest in Drosophila embryos. With slight modifications, the method developed in this work can be applied to many other models of development and other stimulus-response behaviors during development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.