ArticleMolecular biology of the cell2016
Two distinct myosin II populations coordinate ovulatory contraction of the myoepithelial sheath in the Caenorhabditis elegans somatic gonad.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Transcriptomic Analysis of the Spatiotemporal Axis of Oogenesis and Fertilization inbioRxiv : the preprint server for biology · 2024Article
- Transcriptomic analysis of the spatiotemporal axis of oogenesis and fertilization inFrontiers in cell and developmental biology · 2024Article
- FLN-1/filamin is required to anchor the actomyosin cytoskeleton and for global organization of sub-cellular organelles in a contractile tissue.Cytoskeleton (Hoboken, N.J.) · 2020Article
- TwoThe Journal of biological chemistry · 2020Article
- Mechanisms that regulate morphogenesis of a highly branched neuron in C. elegans.Developmental biology · 2019Review
- Actin assembly and non-muscle myosin activity drive dendrite retraction in an UNC-6/Netrin dependent self-avoidance response.PLoS genetics · 2019Article
- Regulation of Actin Dynamics in theJournal of developmental biology · 2019Review
- Syncytial germline architecture is actively maintained by contraction of an internal actomyosin corset.Nature communications · 2018Article
- Spectrin regulates cell contractility through production and maintenance of actin bundles in the Caenorhabditis elegans spermatheca.Molecular biology of the cell · 2018Article
- The myosin light-chain kinase MLCK-1 relocalizes during Caenorhabditis elegans ovulation to promote actomyosin bundle assembly and drive contraction.Molecular biology of the cell · 2018Article
- Myosin activity drives actomyosin bundle formation and organization in contractile cells of theMolecular biology of the cell · 2017Article
- Article
- INF2- and FHOD-related formins promote ovulation in the somatic gonad of C. elegans.Cytoskeleton (Hoboken, N.J.) · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The myoepithelial sheath in the somatic gonad of the nematode Caenorhabditis elegans has nonstriated contractile actomyosin networks that produce highly coordinated contractility for ovulation of mature oocytes. Two myosin heavy chains are expressed in the myoepithelial sheath, which are also expressed in the body-wall striated muscle. The troponin/tropomyosin system is also present and essential for ovulation. Therefore, although the myoepithelial sheath has smooth muscle-like contractile apparatuses, it has a striated muscle-like regulatory mechanism through troponin/tropomyosin. Here we report that the myoepithelial sheath has a distinct myosin population containing nonmuscle myosin II isoforms, which is regulated by phosphorylation and essential for ovulation. MLC-4, a nonmuscle myosin regulatory light chain, localizes to small punctate structures and does not colocalize with large, needle-like myosin filaments containing MYO-3, a striated-muscle myosin isoform. RNA interference of MLC-4, as well as of its upstream regulators, LET-502 (Rho-associated coiled-coil forming kinase) and MEL-11 (a myosin-binding subunit of myosin phosphatase), impairs ovulation. Expression of a phosphomimetic MLC-4 mutant mimicking a constitutively active state also impairs ovulation. A striated-muscle myosin (UNC-54) appears to provide partially compensatory contractility. Thus the results indicate that the two spatially distinct myosin II populations coordinately regulate ovulatory contraction of the myoepithelial sheath.
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