ArticleMolecular biology of the cell2016
Confined diffusion of transmembrane proteins and lipids induced by the same actin meshwork lining the plasma membrane.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
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Who cites it
96 citing papers in PubMed, 208 citations in OpenAlex.
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- A single-molecule reporter of membrane-proximal actin detects rapid remodeling upon B cell receptor clustering.bioRxiv : the preprint server for biology · 2026Article
- Spectrin coordinates cell shape and signaling essential for epidermal differentiation.The Journal of cell biology · 2026Article
- Five key challenges for the plasma membrane insertion of nicotinic acetylcholine receptors in cellular model systems.Molecular and cellular biochemistry · 2026Review
- Single-molecule characterization of opioid receptor heterodimers reveals soluble µ-δ dimer blocker peptide alleviates morphine tolerance.Nature communications · 2025Article
- Single-molecule methods for characterizing receptor dimers reveal metastable opioid receptor homodimers that induce functional modulation.Nature communications · 2025Article
- Concurrent diffusion of nicotinic acetylcholine receptors and fluorescent cholesterol disclosed by two-colour sub-millisecond MINFLUX-based single-molecule tracking.Nature communications · 2025Article
- The mechanism of nesprin-2 accumulation at the nucleus front during confined cell migration.Biophysical journal · 2025Article
- Targeted elimination of mesenchymal-like cancer cells through cyclic stretch activation of Piezo1 channels: the physical aspects.Biophysical reviews · 2025Review
- Intracellular pressure controls the propagation of tension in crumpled cell membranes.Nature communications · 2025Article
- Lipid Membrane Domains Control Actin Network Viscoelasticity.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue.Nature communications · 2024Article
- Quantifying biomolecular organisation in membranes with brightness-transit statistics.Nature communications · 2024Article
- Confinement energy landscape classification reveals membrane receptor nano-organization mechanisms.Biophysical journal · 2024Article
- Inhibition of WNK Kinases in NK Cells Disrupts Cellular Osmoregulation and Control of Tumor Metastasis.Journal of innate immunity · 2024Article
- Particle-environment interactions in arbitrary dimensions: A unifying analytic framework to model diffusion with inert spatial heterogeneities.Physical review research · 2023Article
- Synapsin condensation controls synaptic vesicle sequestering and dynamics.Nature communications · 2023Article
- A Survey of Models of Cell Membranes: Toward a New Understanding of Membrane Organization.Cold Spring Harbor perspectives in biology · 2023Review
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms by which the diffusion rate in the plasma membrane (PM) is regulated remain unresolved, despite their importance in spatially regulating the reaction rates in the PM. Proposed models include entrapment in nanoscale noncontiguous domains found in PtK2 cells, slow diffusion due to crowding, and actin-induced compartmentalization. Here, by applying single-particle tracking at high time resolutions, mainly to the PtK2-cell PM, we found confined diffusion plus hop movements (termed "hop diffusion") for both a nonraft phospholipid and a transmembrane protein, transferrin receptor, and equal compartment sizes for these two molecules in all five of the cell lines used here (actual sizes were cell dependent), even after treatment with actin-modulating drugs. The cross-section size and the cytoplasmic domain size both affected the hop frequency. Electron tomography identified the actin-based membrane skeleton (MSK) located within 8.8 nm from the PM cytoplasmic surface of PtK2 cells and demonstrated that the MSK mesh size was the same as the compartment size for PM molecular diffusion. The extracellular matrix and extracellular domains of membrane proteins were not involved in hop diffusion. These results support a model of anchored TM-protein pickets lining actin-based MSK as a major mechanism for regulating diffusion.
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