Evidence map›Paper›PMID 26864539›Full record

ArticleInternational archives of occupational and environmental health2016

Petrol exposure and DNA integrity of peripheral lymphocytes.

R N Naidoo, M H Makwela, A Chuturgoon, C Tiloke, P Ramkaran, A Phulukdaree

Abstract read
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In one paragraph

Article in International archives of occupational and environmental health, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Primary DNA Damage in Dry Cleaners with Perchlorethylene Exposure.The international journal of occupational and environmental medicine · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

R N NaidooDiscipline of Occupational and Environmental Health, School of Nursing and Public Health, University of KwaZulu-Natal, Room 321 George Campbell Building, Durban, 4041, South Africa. naidoon@ukzn.ac.za.
M H MakwelaDiscipline of Occupational and Environmental Health, School of Nursing and Public Health, University of KwaZulu-Natal, Room 321 George Campbell Building, Durban, 4041, South Africa.
A ChuturgoonDiscipline of Medical Biochemistry, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
C TilokeDiscipline of Medical Biochemistry, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
P RamkaranDiscipline of Medical Biochemistry, School of Laboratory Medicine and Medical Sciences, University of KwaZulu-Natal, Durban, South Africa.
A PhulukdareeDepartment of Physiology, School of Medicine, University of Pretoria, Pretoria, South Africa.
University of KwaZulu-Natal · ZAUniversity of Pretoria · ZA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo determine the effect of petrol exposure on DNA integrity in peripheral blood lymphocytes among petrol attendants and a non-exposed comparison population.

methodsThis cross-sectional study included 101 fuel station employees and 50 office-based non-exposed workers in Durban, South Africa. Participants were interviewed using a validated questionnaire. Genomic DNA was extracted from peripheral lymphocytes for the benzo(a)pyrene diol epoxide (BPDE)-DNA adduct assay (ELISA), and DNA damage was determined using the comet assay and reported as percentage tail DNA.

resultsThe exposed (n = 101) and non-exposed participants (n = 50) varied with regard to age, housing, smoking, and proximity to industry and petrol stations. Among the exposed, the mean duration of employment in the fuel industry was 5.8 years (SD = 4.6), and among those pumping fuel (n = 75), the mean metric tons of petrol pumped in the past 12 months per worker was 199.2 (SD = 88.9). The mean percentage tail DNA varied significantly between exposed and non-exposed groups: 23.8 % (SD = 13.3) and 8.1 % (SD = 1.8) (p < 0.01), respectively. A significant difference existed between the groups for BPDE-DNA adducts: 30.0 ng/ml (SD = 12.7) and 18.1 ng/ml (SD = 18.2) (p < 0.0001), respectively. Regression models, adjusting for cigarette smoking, age, and sex, showed a 16.5 greater percentage tail DNA among the exposed compared to non-exposed (95 % CI 11.8-21.1 %), while the exposed group had a 12.9 ng/ml greater increase in BPDE-DNA adducts has compared to the unexposed (95 % CI 7.2-18.7 ng/ml). Cigarette smoking resulted in almost a 3.5 % increase in percentage tail DNA.

conclusionOur study adds to the literature that long-term, low-dose exposure to vehicular fuels is likely to result in altered DNA integrity and genotoxicity among petrol attendants. These results strengthen the case that these workers must be afforded appropriate protection to prevent serious adverse outcomes.

Indexed as

DNA Damage7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideAdultComet AssayCross-Sectional StudiesDNADNA AdductsFemaleHumansIndustryLymphocytesMaleMiddle AgedOccupational ExposurePetroleumPetroleum Pollution7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxidebenzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNADNADNA AdductsPetroleumBPDE-DNA adductComet assayDNA damagePetrol attendants

Identifiers

PMID26864539
OpenAlexW2264689115

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.