Evidence map›Paper›PMID 26864394›Full record

ArticleChemical communications (Cambridge, England)2016

The one-pot multienzyme (OPME) synthesis of human blood group H antigens and a human milk oligosaccharide (HMOS) with highly active Thermosynechococcus elongates α1-2-fucosyltransferase.

Chao Zhao, Yijing Wu, Hai Yu, Ishita M Shah, Yanhong Li, Jie Zeng, Bin Liu, David A Mills, Xi Chen

Abstract read
In one paragraph

Article in Chemical communications (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Article
  3. HPLC-Based Automated Synthesis of Glycans in Solution.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022
    Article
  4. Food chemistry: X · 2022
    Article
  5. Article
  6. Fucosylated oligosaccharide Lacto-Food chemistry: X · 2022
    Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Biosynthesis of Raffinose and Stachyose from Sucrose via anApplied and environmental microbiology · 2019
    Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chao ZhaoCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Yijing Wu
Hai Yu
Ishita M Shah
Yanhong Li
Jie Zeng
Bin Liu
David A Mills
Xi Chen

Funding

Oligosaccharides as antimicrobials and prebioticsR01HD065122 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI CHEN, XI · 2010 to 2013
$1.8M
NICHD NIH HHS R01 HD065122NICHD NIH HHS R01HD065122
6 · The paper itself

Abstract

A novel α1-2-fucosyltransferase from Thermosynechococcus elongatus BP-1 (Te2FT) with high fucosyltransferase activity and low donor hydrolysis activity was discovered and characterized. It was used in an efficient one-pot multienzyme (OPME) fucosylation system for the high-yield synthesis of human blood group H antigens containing β1-3-linked galactosides and an important human milk oligosaccharide (HMOS) lacto-N-fucopentaose I (LNFP I) on preparative and gram scales. LNFP I was shown to be selectively consumed by Bifidobacterium longum subsp. infantis but not Bifidobacterium animalis subsp. lactis and is a potential prebiotic.

Indexed as

Blood Group AntigensCarbohydrate ConformationCyanobacteriaEnzyme ActivationFucosyltransferasesGalactoside 2-alpha-L-fucosyltransferaseHumansMilk, HumanOligosaccharidesBlood Group AntigensFucosyltransferasesGalactoside 2-alpha-L-fucosyltransferaseOligosaccharides

Identifiers

PMID26864394
PMCPMC4775349

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.