ArticleBMC plant biology2016
Revisiting chromatin binding of the Arabidopsis UV-B photoreceptor UVR8.
Article in BMC plant biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 36 citations in OpenAlex.
- A redox-regulated RCC1-like protein controls catalase activity in Arabidopsis.Nature communications · 2026Article
- Plant responses to UV-B radiation: signaling, acclimation and stress tolerance.Stress biology · 2022Review
- Activation and negative feedback regulation of SlHY5 transcription by the SlBBX20/21-SlHY5 transcription factor module in UV-B signaling.The Plant cell · 2022Article
- ELONGATED HYPOCOTYL5 Negatively RegulatesPlant physiology · 2020Article
- Degradation of the transcription factors PIF4 and PIF5 under UV-B promotes UVR8-mediated inhibition of hypocotyl growth in Arabidopsis.The Plant journal : for cell and molecular biology · 2020Article
- Ultraviolet Radiation From a Plant Perspective: The Plant-Microorganism Context.Frontiers in plant science · 2020Review
- Plant photoreceptors and their signaling components compete for COP1 binding via VP peptide motifs.The EMBO journal · 2019Article
- Arabidopsis CRL4 Complexes: Surveying Chromatin States and Gene Expression.Frontiers in plant science · 2019Review
- Plant Chromatin Catches the Sun.Frontiers in plant science · 2019Review
- Tomato UV-B receptor SlUVR8 mediates plant acclimation to UV-B radiation and enhances fruit chloroplast development via regulating SlGLK2.Scientific reports · 2018Article
- UV RESISTANCE LOCUS 8 FromFrontiers in plant science · 2018Article
- Environmental effects of ozone depletion and its interactions with climate change: Progress report, 2016.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2017Article
- Regulation of transcription by the Arabidopsis UVR8 photoreceptor involves a specific histone modification.Plant molecular biology · 2016Article
- SHORT HYPOCOTYL1 Encodes a SMARCA3-Like Chromatin Remodeling Factor Regulating Elongation.Plant physiology · 2016Article
- COP1 is required for UV-B-induced nuclear accumulation of the UVR8 photoreceptor.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlants perceive UV-B through the UV RESISTANCE LOCUS 8 (UVR8) photoreceptor and UVR8 activation leads to changes in gene expression such as those associated with UV-B acclimation and stress tolerance. Albeit functionally unrelated, UVR8 shows some homology with RCC1 (Regulator of Chromatin Condensation 1) proteins from non-plant organisms at the sequence level. These proteins act as guanine nucleotide exchange factors for Ran GTPases and bind chromatin via histones. Subsequent to the revelation of this sequence homology, evidence was presented showing that UVR8 activity involves interaction with chromatin at the loci of some target genes through histone binding. This suggested a UVR8 mode-of-action intimately and directly linked with gene transcription. However, several aspects of UVR8 chromatin association remained undefined, namely the impact of UV-B on the process and how UVR8 chromatin association related to the transcription factor ELONGATED HYPOCOTYL 5 (HY5), which is important for UV-B signalling and has overlapping chromatin targets. Therefore, we have investigated UVR8 chromatin association in further detail.
resultsUnlike the claims of previous studies, our chromatin immunoprecipitation (ChIP) experiments do not confirm UVR8 chromatin association. In contrast to human RCC1, recombinant UVR8 also does not bind nucleosomes in vitro. Moreover, fusion of a VP16 activation domain to UVR8 did not alter expression of proposed UVR8 target genes in transient gene expression assays. Finally, comparison of the Drosophila DmRCC1 and the Arabidopsis UVR8 crystal structures revealed that critical histone- and DNA-interaction residues apparent in DmRCC1 are not conserved in UVR8.
conclusionThis has led us to conclude that the cellular activity of UVR8 likely does not involve its specific binding to chromatin at target genes.
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