ArticleScientific reports2016
Alp7/TACC-Alp14/TOG generates long-lived, fast-growing MTs by an unconventional mechanism.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Microtubules in Microorganisms: How Tubulin Isotypes Contribute to Diverse Cytoskeletal Functions.Frontiers in cell and developmental biology · 2022Review
- Human Cytomegalovirus Exploits TACC3 To Control Microtubule Dynamics and Late Stages of Infection.Journal of virology · 2021Article
- Kinesin-6 Klp9 orchestrates spindle elongation by regulating microtubule sliding and growth.eLife · 2021Article
- XMAP215 and γ-tubulin additively promote microtubule nucleation in purified solutions.Molecular biology of the cell · 2020Article
- Insights into allosteric control of microtubule dynamics from a buried β-tubulin mutation that causes faster growth and slower shrinkage.Protein science : a publication of the Protein Society · 2020Article
- Review
- Behaviors of individual microtubules and microtubule populations relative to critical concentrations: dynamic instability occurs when critical concentrations are driven apart by nucleotide hydrolysis.Molecular biology of the cell · 2020Article
- TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells.Cell reports · 2020Article
- Microtubule polymerase and processive plus-end tracking functions originate from distinct features within TOG domain arrays.Molecular biology of the cell · 2019Article
- Suppressor Analysis Uncovers That MAPs and Microtubule Dynamics Balance with the Cut7/Kinesin-5 Motor for Mitotic Spindle Assembly inG3 (Bethesda, Md.) · 2019Article
- Measuring microtubule dynamics.Essays in biochemistry · 2018Review
- Microtubule dynamics: an interplay of biochemistry and mechanics.Nature reviews. Molecular cell biology · 2018Review
- The XMAP215 Ortholog Alp14 Promotes Microtubule Nucleation in Fission Yeast.Current biology : CB · 2018Article
- Nucleotide- and Mal3-dependent changes in fission yeast microtubules suggest a structural plasticity view of dynamics.Nature communications · 2017Article
- A microtubule polymerase cooperates with the kinesin-6 motor and a microtubule cross-linker to promote bipolar spindle assembly in the absence of kinesin-5 and kinesin-14 in fission yeast.Molecular biology of the cell · 2017Article
- Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots.Chemical Society reviews · 2017Review
- Purification and characterisation of the fission yeast Ndc80 complex.Protein expression and purification · 2017Article
- An unconventional interaction between Dis1/TOG and Mal3/EB1 in fission yeast promotes the fidelity of chromosome segregation.Journal of cell science · 2016Article
- Xenopus TACC2 is a microtubule plus end-tracking protein that can promote microtubule polymerization during embryonic development.Molecular biology of the cell · 2016Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Alp14 is a TOG-family microtubule polymerase from S. pombe that tracks plus ends and accelerates their growth. To interrogate its mechanism, we reconstituted dynamically unstable single isoform S. pombe microtubules with full length Alp14/TOG and Alp7, the TACC-family binding partner of Alp14. We find that Alp14 can drive microtubule plus end growth at GTP-tubulin concentrations at least 10-fold below the usual critical concentration, at the expense of increased catastrophe. This reveals Alp14 to be a highly unusual enzyme that biases the equilibrium for the reaction that it catalyses. Alp7/TACC enhances the effectiveness of Alp14, by increasing its occupancy. Consistent with this, we show in live cells that Alp7 deletion produces very similar MT dynamics defects to Alp14 deletion. The ability of Alp7/14 to accelerate and bias GTP-tubulin exchange at microtubule plus ends allows it to generate long-lived, fast-growing microtubules at very low cellular free tubulin concentrations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.