ArticleJournal of applied genetics2016
Enhanced resistance to Sclerotinia sclerotiorum in Brassica napus by co-expression of defensin and chimeric chitinase genes.
Article in Journal of applied genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 45 citations in OpenAlex.
- White Mold: A Global Threat to Crops and Key Strategies for Its Sustainable Management.Microorganisms · 2024Review
- Small Cationic Cysteine-Rich Defensin-Derived Antifungal Peptide Controls White Mold in Soybean.Journal of fungi (Basel, Switzerland) · 2023Article
- Fungal effectors versus defense-related genes ofFrontiers in plant science · 2023Review
- The Characterization of theInternational journal of molecular sciences · 2022Article
- Use of Defensins to Develop Eco-Friendly Alternatives to Synthetic Fungicides to Control Phytopathogenic Fungi and Their Mycotoxins.Journal of fungi (Basel, Switzerland) · 2022Review
- Tackling Control of a Cosmopolitan Phytopathogen:Frontiers in plant science · 2021Review
- Genomics Armed With Diversity Leads the Way inFrontiers in genetics · 2021Review
- Development of TransgenicPlants (Basel, Switzerland) · 2020Review
- Transcriptomic Analysis Reveals Candidate Genes Responsive toInternational journal of molecular sciences · 2020Article
- Effect of Introducing Chitinase Gene on the Resistance of Tuber Mustard against White Mold.The plant pathology journal · 2020Article
- Molecular and genetic analysis of defensive responses of Brassica juncea - B. fruticulosa introgression lines to Sclerotinia infection.Scientific reports · 2019Article
- Current Status and Challenges in Identifying Disease Resistance Genes inFrontiers in plant science · 2017Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the major fungal diseases of Brassica napus L. To develop resistance against this fungal disease, the defensin gene from Raphanus sativus and chimeric chit42 from Trichoderma atroviride with a C-terminal fused chitin-binding domain from Serratia marcescens were co-expressed in canola via Agrobacterium-mediated transformation. Twenty transformants were confirmed to carry the two transgenes as detected by polymerase chain reaction (PCR), with 4.8 % transformation efficiency. The chitinase activity of PCR-positive transgenic plants were measured in the presence of colloidal chitin, and five transgenic lines showing the highest chitinase activity were selected for checking the copy number of the transgenes through Southern blot hybridisation. Two plants carried a single copy of the transgenes, while the remainder carried either two or three copies of the transgenes. The antifungal activity of two transgenic lines that carried a single copy of the transgenes (T4 and T10) was studied by a radial diffusion assay. It was observed that the constitutive expression of these transgenes in the T4 and T10 transgenic lines suppressed the growth of S. sclerotiorum by 49 % and 47 %, respectively. The two transgenic lines were then let to self-pollinate to produce the T
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