ArticleScientific reports2016
An Efficient LCM-Based Method for Tissue Specific Expression Analysis of Genes and miRNAs.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
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- Fusion of Wild-Type Mesoangioblasts with Myotubes of mtDNA Mutation Carriers Leads to a Proportional Reduction in mtDNA Mutation Load.International journal of molecular sciences · 2023Article
- OMICs, Epigenetics, and Genome Editing Techniques for Food and Nutritional Security.Plants (Basel, Switzerland) · 2021Review
- Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based Method.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Recent technological advancements in stem cell research for targeted therapeutics.Drug delivery and translational research · 2020Review
- CLAVATA signaling pathway genes modulating flowering time and flower number in chickpea.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Laser-capture microdissection of murine lung for differential cellular RNA analysis.Cell and tissue research · 2019Article
- An Overview of Methodologies in Studying lncRNAs in the High-Throughput Era: When Acronyms ATTACK!Methods in molecular biology (Clifton, N.J.) · 2019Article
- A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms.Plant methods · 2019Article
- A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis.Plant methods · 2019Article
- Plant small RNAs: advancement in the understanding of biogenesis and role in plant development.Planta · 2018Review
- An Insight into the Difficulties in the Discovery of Specific Biomarkers of Limbal Stem Cells.International journal of molecular sciences · 2018Review
- Expression dynamics of miRNAs and their targets in seed germination conditions reveals miRNA-ta-siRNA crosstalk as regulator of seed germination.Scientific reports · 2018Article
- Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Phytohormonal crosstalk modulates the expression of miR166/165s, target Class III HD-ZIPs, and KANADI genes during root growth in Arabidopsis thaliana.Scientific reports · 2017Article
- Genome-wide analysis of transcription factors during somatic embryogenesis in banana (Musa spp.) cv. Grand Naine.PloS one · 2017Article
- Root transcriptome of two contrasting indica rice cultivars uncovers regulators of root development and physiological responses.Scientific reports · 2016Article
- Article
- Identification of key pathways and genes in colorectal cancer using bioinformatics analysis.Medical oncology (Northwood, London, England) · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Laser Capture Microdissection (LCM) is a powerful tool to isolate and study gene expression pattern of desired and less accessible cells or tissues from a heterogeneous population. Existing LCM-based methods fail to obtain high quality RNA including small RNAs from small microdissected plant tissue and therefore, are not suitable for miRNA expression studies. Here, we describe an efficient and cost-effective method to obtain both high quality RNA and miRNAs from LCM-derived embryonic root apical meristematic tissue, which is difficult to access. We have significantly modified and improved the tissue fixation, processing, sectioning and RNA isolation steps and minimized the use of kits. Isolated RNA was checked for quality with bioanalyzer and used for gene expression studies. We have confirmed the presence of 19-24 nucleotide long mature miRNAs using modified stem-loop RT-PCR. This modified LCM-based method is suitable for tissue specific expression analysis of both genes and small RNAs (miRNAs).
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