ArticleNature communications2016
Nanocaged enzymes with enhanced catalytic activity and increased stability against protease digestion.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 126 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
126 citing papers in PubMed.
- Programmable DNA Assemblies Reconstitute Supramolecular Protein Function.bioRxiv : the preprint server for biology · 2026Article
- Enhancing Enzyme Activity Via Noncovalent Association with DNA-Functionalized Nanoparticles.JACS Au · 2026Article
- DNA-Enzyme Hybrid Nanostructures: Functional Materials to Modulate Enzymatic Activity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Construction of a COChemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Peptide-based artificial cytoskeleton enhances colocalized cascade reactions in cell-like microreactors.Chemical science · 2026Article
- Extreme sensitivity label-free biosensing platform based on topologically disruptive phase nano-optics.Microsystems & nanoengineering · 2026Article
- Biological Nanoscaffolds from Hierarchical Construction to Applications.Molecules (Basel, Switzerland) · 2026Review
- Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.Molecules (Basel, Switzerland) · 2026Review
- DNA Origami and Its Applications in Synthetic Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Recent advances in engineering nano/microrobots for tumor treatment.Acta pharmaceutica Sinica. B · 2025Review
- Article
- Discovery of Surface-Engineered Nanoparticles That Boost Enzyme Activity via High-Throughput Screening and Machine Learning.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Analyzing DNA Origami Nanostructure Assembly by Dynamic Light Scattering and Nanoparticle Tracking Analysis.Small methods · 2025Article
- Enzyme Reactions Are Accelerated or Decelerated When the Enzymes Are Located Near the DNA Nanostructure.ACS applied materials & interfaces · 2025Article
- Nucleic Acid Framework-Enabled Spatial Organization for Biological Applications.Chem & bio engineering · 2025Review
- Droplet-supported liquid-liquid lateral phase separation as a step to floating protein heterostructures.Nature communications · 2025Article
- A DNA origami-based enzymatic cascade nanoreactor for chemodynamic cancer therapy and activation of antitumor immunity.Science advances · 2025Article
- A modular DNA origami nanocompartment for engineering a cell-free, protein unfolding and degradation pathway.Nature nanotechnology · 2024Article
- Organ-Specific Gene Expression Control Using DNA Origami-Based Nanodevices.Nano letters · 2024Article
- Boosted Enzyme Activity via Encapsulation within Metal-Organic Frameworks with Pores Matching Enzyme Size and Shape.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
66 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Cells routinely compartmentalize enzymes for enhanced efficiency of their metabolic pathways. Here we report a general approach to construct DNA nanocaged enzymes for enhancing catalytic activity and stability. Nanocaged enzymes are realized by self-assembly into DNA nanocages with well-controlled stoichiometry and architecture that enabled a systematic study of the impact of both encapsulation and proximal polyanionic surfaces on a set of common metabolic enzymes. Activity assays at both bulk and single-molecule levels demonstrate increased substrate turnover numbers for DNA nanocage-encapsulated enzymes. Unexpectedly, we observe a significant inverse correlation between the size of a protein and its activity enhancement. This effect is consistent with a model wherein distal polyanionic surfaces of the nanocage enhance the stability of active enzyme conformations through the action of a strongly bound hydration layer. We further show that DNA nanocages protect encapsulated enzymes against proteases, demonstrating their practical utility in functional biomaterials and biotechnology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.