ArticleSensors (Basel, Switzerland)2016
Evaluating Quantum Dot Performance in Homogeneous FRET Immunoassays for Prostate Specific Antigen.
Article in Sensors (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 43 citations in OpenAlex.
- Dual Quantum Dot Molecular FRET Probes for Picomolar DNA Hexaplexing.Small methods · 2026Article
- Objective clustering protocol for single-molecule data: A lifetime vs. intensity study.Biophysical reports · 2026Article
- Quantum Dot-Enabled Biosensing for Prostate Cancer Diagnostics.Nanomaterials (Basel, Switzerland) · 2025Review
- Fluorescence Multi-Detection Device Using a Lensless Matrix Addressable microLED Array.Biosensors · 2024Article
- Triplexed CEA-NSE-PSA Immunoassay Using Time-Gated Terbium-to-Quantum Dot FRET.Molecules (Basel, Switzerland) · 2020Article
- FRET as a biomolecular research tool - understanding its potential while avoiding pitfalls.Nature methods · 2019Review
- In Vivo Biosensing Using Resonance Energy Transfer.Biosensors · 2019Review
- Sensing with photoluminescent semiconductor quantum dots.Methods and applications in fluorescence · 2019Article
- A DNA-Based Assay for Digoxin Detection.Biosensors · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The integration of semiconductor quantum dots (QDs) into homogeneous Förster resonance energy transfer (FRET) immunoassay kits for clinical diagnostics can provide significant advantages concerning multiplexing and sensitivity. Here we present a facile and functional QD-antibody conjugation method using three commercially available QDs with different photoluminescence (PL) maxima (605 nm, 655 nm, and 705 nm). The QD-antibody conjugates were successfully applied for FRET immunoassays against prostate specific antigen (PSA) in 50 µL serum samples using Lumi4-Tb (Tb) antibody conjugates as FRET donors and time-gated PL detection on a KRYPTOR clinical plate reader. Förster distance and Tb donor background PL were directly related to the analytical sensitivity for PSA, which resulted in the lowest limits of detection for Tb-QD705 (2 nM), followed by Tb-QD655 (4 nM), and Tb-QD605 (23 nM). Duplexed PSA detection using the Tb-QD655 and Tb-QD705 FRET-pairs demonstrated the multiplexing ability of our immunoassays. Our results show that FRET based on QD acceptors is suitable for multiplexed and sensitive biomarker detection in clinical diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.