ArticleScientific reports2016
Highly efficient optogenetic cell ablation in C. elegans using membrane-targeted miniSOG.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
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- Insulin and epidermal signals independently shape sexually dimorphic neurite branching in C. elegans.EMBO reports · 2025Article
- Genetic approaches for targeted oxidative stress.NAR cancer · 2025Review
- Excretory gland cell and NSPC genes in C. elegans: investigating their physiological roles.Genetics · 2025Article
- Calcium levels in ASER neurons determine behavioral valence by engaging distinct neuronal circuits in C. elegans.Nature communications · 2025Article
- Suppressing APOE4-induced neural pathologies by targeting the VHL-HIF axis.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
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- PIEZO acts in an intestinal valve to regulate swallowing in C. elegans.Nature communications · 2024Article
- Suppressing APOE4-induced mortality and cellular damage by targeting VHL.bioRxiv : the preprint server for biology · 2024Article
- Laboratory Evolution of Metalloid Reductase Substrate Recognition and Nanoparticle Product Size.ACS chemical biology · 2024Article
- Molecular and circuit mechanisms underlying avoidance of rapid cooling stimuli in C. elegans.Nature communications · 2024Article
- A cGAL-UAS bipartite expression toolkit forProceedings of the National Academy of Sciences of the United States of America · 2023Article
- UNC-49 is a redox-sensitive GABAScience advances · 2023Article
- The head mesodermal cell couples FMRFamide neuropeptide signaling with rhythmic muscle contraction in C. elegans.Nature communications · 2023Article
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- Optogenetic polymerization and assembly of electrically functional polymers for modulation of single-neuron excitability.Science advances · 2022Article
- Multisensory Integration inBrain sciences · 2022Review
- Targeting Mitochondrial Function with Chemoptogenetics.Biomedicines · 2022Review
Corrections and comments
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Authors and funding
2 authors.
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Abstract
The genetically encoded photosensitizer miniSOG (mini Singlet Oxygen Generator) can be used to kill cells in C. elegans. miniSOG generates the reactive oxygen species (ROS) singlet oxygen after illumination with blue light. Illumination of neurons expressing miniSOG targeted to the outer mitochondrial membrane (mito-miniSOG) causes neuronal death. To enhance miniSOG's efficiency as an ablation tool in multiple cell types we tested alternative targeting signals. We find that membrane targeted miniSOG allows highly efficient cell killing. When combined with a point mutation that increases miniSOG's ROS generation, membrane targeted miniSOG can ablate neurons in less than one tenth the time of mito-miniSOG. We extend the miniSOG ablation technique to non-neuronal tissues, revealing an essential role for the epidermis in locomotion. These improvements expand the utility and throughput of optogenetic cell ablation in C. elegans.
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