Evidence map›Paper›PMID 26860281›Full record

ArticleJournal of controlled release : official journal of the Controlled Release Society2016

Microbubbles and ultrasound increase intraventricular polyplex gene transfer to the brain.

James-Kevin Y Tan, Binhan Pham, Yujin Zong, Camilo Perez, Don O Maris, Ashton Hemphill, Carol H Miao, Thomas J Matula, Pierre D Mourad, Hua Wei and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of controlled release : official journal of the Controlled Release Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Review
  13. Review
  14. Polyplex transfection from intracerebroventricular delivery is not significantly affected by traumatic brain injury.Journal of controlled release : official journal of the Controlled Release Society · 2020
    Article
  15. Article
  16. Article
  17. Article
  18. Applications of Ultrasound to Stimulate Therapeutic Revascularization.International journal of molecular sciences · 2019
    Review
  19. Antitumor activity of integrin αTranslational cancer research · 2019
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

James-Kevin Y TanDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, USA.
Binhan PhamDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, USA.
Yujin ZongCenter for Industrial and Medical Ultrasound, University of Washington, Seattle, WA 98195, USA; Department of Biomedical Engineering, Xian Jiaotong University, Xi'an, 710049, China.
Camilo PerezDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Center for Industrial and Medical Ultrasound, University of Washington, Seattle, WA 98195, USA.
Don O MarisDepartment of Neurological Surgery, University of Washington, Seattle, WA 98109, USA.
Ashton HemphillDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, USA.
Carol H MiaoDepartment of Pediatrics, University of Washington, Seattle, WA 98195, USA.
Thomas J MatulaCenter for Industrial and Medical Ultrasound, University of Washington, Seattle, WA 98195, USA.
Pierre D MouradDepartment of Neurological Surgery, University of Washington, Seattle, WA 98109, USA.
Hua WeiDepartment of Chemistry, Lanzhou University, Lanzhou 730000, China.
Drew L SellersDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, USA.
Philip J HornerDepartment of Neurological Surgery, University of Washington, Seattle, WA 98109, USA. Electronic address: pjhorner@houstonmethodist.org.
Suzie H PunDepartment of Bioengineering, University of Washington, Seattle, WA 98195, USA; Molecular Engineering and Sciences Institute, University of Washington, Seattle, WA 98195, USA. Electronic address: spun@uw.edu.

Funding

Ultrasound-aided gene transfer to direct cortical neurogenesis after brain injuryR01NS064404 · NINDS · UNIVERSITY OF WASHINGTON · PI HORNER, PHILIP J, PUN, SUZIE H. · 2009 to 2018
$3.5M
Development of clinically feasible Ultrasound-mediated gene therapy for hemophiliaR01HL128139 · NHLBI · SEATTLE CHILDREN'S HOSPITAL · PI MIAO, CAROL H · 2015 to 2018
$1.9M
Combined stem cell transplantation and targeted microstimulation to direct the foR01NS066357 · NINDS · UNIVERSITY OF WASHINGTON · PI HORNER, PHILIP J, MORITZ, CHET T. · 2009 to 2012
$1.3M
NHLBI NIH HHS R01 HL128139NINDS NIH HHS R01 NS064404NINDS NIH HHS R01 NS066357
6 · The paper itself

Abstract

Neurons in the brain can be damaged or lost from neurodegenerative disease, stroke, or traumatic injury. Although neurogenesis occurs in mammalian adult brains, the levels of natural neurogenesis are insufficient to restore function in these cases. Gene therapy has been pursued as a promising strategy to induce differentiation of neural progenitor cells into functional neurons. Non-viral vectors are a preferred method of gene transfer due to potential safety and manufacturing benefits but suffer from lower delivery efficiencies compared to viral vectors. Since the neural stem and progenitor cells reside in the subventricular zone of the brain, intraventricular injection has been used as an administration route for gene transfer to these cells. However, the choroid plexus epithelium remains an obstacle to delivery. Recently, transient disruption of the blood-brain barrier by microbubble-enhanced ultrasound has been used to successfully improve drug delivery to the brain after intravenous injection. In this work, we demonstrate that microbubble-enhanced ultrasound can similarly improve gene transfer to the subventricular zone after intraventricular injection. Microbubbles of different surface charges (neutral, slightly cationic, and cationic) were prepared, characterized by acoustic flow cytometry, and evaluated for their ability to increase the permeability of immortalized choroid plexus epithelium monolayers in vitro. Based on these results, slightly cationic microbubbles were evaluated for microbubble and ultrasound-mediated enhancement of non-viral gene transfer in vivo. When coupled with our previously reported gene delivery vehicles, the slightly cationic microbubbles significantly increased ultrasound-mediated transfection of the murine brain when compared to commercially available Definity® microbubbles. Temporary disruption of the choroid plexus by microbubble-enhanced ultrasound is therefore a viable way of enhancing gene delivery to the brain and merits further research.

Indexed as

Gene Transfer TechniquesAnimalsBlood-Brain BarrierBrainCationsCell LineChoroid PlexusDrug Delivery SystemsFemaleGenetic TherapyHumansInjections, IntraventricularMice, Inbred C57BLMicrobubblesPermeabilitySurface PropertiesCationsChoroid plexus epitheliumGene deliveryIn vivoMicrobubblePolyplexUltrasound

Identifiers

PMID26860281
PMCPMC4944389

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.