ArticlePloS one2016
DNA Inversion Regulates Outer Membrane Vesicle Production in Bacteroides fragilis.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Dynamics of phage-host interactions in Bacteroides fragilis resolved by single-cell transcriptomics.Nature communications · 2026Article
- Lessons from the model gut BacteroidalesJournal of bacteriology · 2025Review
- Gut phages and their interactions with bacterial and mammalian hosts.Journal of bacteriology · 2025Review
- Combinatorial phenotypic landscape enables bacterial resistance to phage infection.bioRxiv : the preprint server for biology · 2025Article
- Role of phase-variable Mfa1 fimbriae ofGut microbes reports · 2025Article
- Cross - Kingdom Dialogue of Microbial Messengers: Multi - Target Regulatory Mechanisms and Therapeutic Strategies of Gut Microbiota - Derived Extracellular Vesicles in Metabolic Diseases.International journal of nanomedicine · 2025Review
- Microbial Extracellular Vesicles in Host-Microbiota Interactions.Results and problems in cell differentiation · 2024Review
- Environmental and Taxonomic Drivers of Bacterial Extracellular Vesicle Production in Marine Ecosystems.Applied and environmental microbiology · 2023Article
- From trash to treasure: the role of bacterial extracellular vesicles in gut health and disease.Frontiers in immunology · 2023Review
- Article
- Long-term persistence of crAss-like phage crAss001 is associated with phase variation in Bacteroides intestinalis.BMC biology · 2021Article
- Isolation and characterisation of ΦcrAss002, a crAss-like phage from the human gut that infects Bacteroides xylanisolvens.Microbiome · 2021Article
- Site-Specific Recombination - How Simple DNA Inversions Produce Complex Phenotypic Heterogeneity in Bacterial Populations.Trends in genetics : TIG · 2021Review
- Analysis of a phase-variable restriction modification system of the human gut symbiont Bacteroides fragilis.Nucleic acids research · 2020Article
- Shining Light on Human Gut Bacteriophages.Frontiers in cellular and infection microbiology · 2020Review
- Molecular Mechanisms ofJournal of bacteriology · 2019Article
- Invertible promoters mediate bacterial phase variation, antibiotic resistance, and host adaptation in the gut.Science (New York, N.Y.) · 2019Article
- Bacterial outer membrane vesicles, a potential vaccine candidate in interactions with host cells based.Diagnostic pathology · 2018Review
- Targeting and inactivation of bacterial toxins by human defensins.Biological chemistry · 2017Review
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phase changes in Bacteroides fragilis, a member of the human colonic microbiota, mediate variations in a vast array of cell surface molecules, such as capsular polysaccharides and outer membrane proteins through DNA inversion. The results of the present study show that outer membrane vesicle (OMV) formation in this anaerobe is also controlled by DNA inversions at two distantly localized promoters, IVp-I and IVp-II that are associated with extracellular polysaccharide biosynthesis and the expression of outer membrane proteins. These promoter inversions are mediated by a single tyrosine recombinase encoded by BF2766 (orthologous to tsr19 in strain NCTC9343) in B. fragilis YCH46, which is located near IVp-I. A series of BF2766 mutants were constructed in which the two promoters were locked in different configurations (IVp-I/IVp-II = ON/ON, OFF/OFF, ON/OFF or OFF/ON). ON/ON B. fragilis mutants exhibited hypervesiculating, whereas the other mutants formed only a trace amount of OMVs. The hypervesiculating ON/ON mutants showed higher resistance to treatment with bile, LL-37, and human β-defensin 2. Incubation of wild-type cells with 5% bile increased the population of cells with the ON/ON genotype. These results indicate that B. fragilis regulates the formation of OMVs through DNA inversions at two distantly related promoter regions in response to membrane stress, although the mechanism underlying the interplay between the two regions controlled by the invertible promoters remains unknown.
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