Evidence map›Paper›PMID 26858940›Full record

ArticleFrontiers in oncology2016

Impact of Freezing Delay Time on Tissue Samples for Metabolomic Studies.

Tonje H Haukaas, Siver A Moestue, Riyas Vettukattil, Beathe Sitter, Santosh Lamichhane, Remedios Segura, Guro F Giskeødegård, Tone F Bathen

Abstract read
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In one paragraph

Article in Frontiers in oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed.

  1. Fingerprinting the Metabolic and Lipidomic Landscapes of Pancreatic Ductal Adenocarcinoma with MALDI Imaging.Clinical cancer research : an official journal of the American Association for Cancer Research · 2026
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  16. Impact of post-surgical freezing delay on brain tumor metabolomics.Metabolomics : Official journal of the Metabolomic Society · 2019
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Tonje H HaukaasDepartment of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; Faculty of Medicine, K. G. Jebsen Center for Breast Cancer Research, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
Siver A MoestueDepartment of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
Riyas VettukattilDepartment of Circulation and Medical Imaging, Norwegian University of Science and Technology , Trondheim , Norway.
Beathe SitterDepartment of Health Science, Faculty of Health and Social Science, Sør-Trøndelag University College , Trondheim , Norway.
Santosh LamichhaneDepartment of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; Department of Food Science, Faculty of Science and Technology, Aarhus University, Årslev, Denmark.
Remedios SeguraMetabolomic and Molecular Image Laboratory, Health Research Institute INCLIVA , Valencia , Spain.
Guro F GiskeødegårdDepartment of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
Tone F BathenDepartment of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trondheim, Norway; Faculty of Medicine, K. G. Jebsen Center for Breast Cancer Research, Institute of Clinical Medicine, University of Oslo, Oslo, Norway.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionMetabolic profiling of intact tumor tissue by high-resolution magic angle spinning (HR MAS) MR spectroscopy (MRS) provides important biological information possibly useful for clinical diagnosis and development of novel treatment strategies. However, generation of high-quality data requires that sample handling from surgical resection until analysis is performed using systematically validated procedures. In this study, we investigated the effect of postsurgical freezing delay time on global metabolic profiles and stability of individual metabolites in intact tumor tissue. MATERIALS AND

methodsTumor tissue samples collected from two patient-derived breast cancer xenograft models (n = 3 for each model) were divided into pieces that were snap-frozen in liquid nitrogen at 0, 15, 30, 60, 90, and 120 min after surgical removal. In addition, one sample was analyzed immediately, representing the metabolic profile of fresh tissue exposed neither to liquid nitrogen nor to room temperature. We also evaluated the metabolic effect of prolonged spinning during the HR MAS experiments in biopsies from breast cancer patients (n = 14). All samples were analyzed by proton HR MAS MRS on a Bruker Avance DRX600 spectrometer, and changes in metabolic profiles were evaluated using multivariate analysis and linear mixed modeling.

resultsMultivariate analysis showed that the metabolic differences between the two breast cancer models were more prominent than variation caused by freezing delay time. No significant changes in levels of individual metabolites were observed in samples frozen within 30 min of resection. After this time point, levels of choline increased, whereas ascorbate, creatine, and glutathione (GS) levels decreased. Freezing had a significant effect on several metabolites but is an essential procedure for research and biobank purposes. Furthermore, four metabolites (glucose, glycine, glycerophosphocholine, and choline) were affected by prolonged HR MAS experiment time possibly caused by physical release of metabolites caused by spinning or due to structural degradation processes.

conclusionThe MR metabolic profiles of tumor samples are reproducible and robust to variation in postsurgical freezing delay up to 30 min.

Indexed as

cancerdegradationfreezing time delayHR MASmetabolic profilemetabolomicsMR spectroscopysnap-freezing

Identifiers

PMID26858940
PMCPMC4730796

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.