ArticleCell medicine2015
Spheroid Formation and Evaluation of Hepatic Cells in a Three-Dimensional Culture Device.
Article in Cell medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The effect of rocking speed and cell inoculation density on hepatic spheroid size: A simple method for size modulation.Bioengineering & translational medicine · 2026Article
- Prospective Assessment of Embryoid Body by Deep Learning on Label-Free Time-Lapse Images from the Microwell Array.Biomedicines · 2026Article
- Fabrication Method for Shape-Controlled 3D Tissue Using High-Porosity Porous Structure.Bioengineering (Basel, Switzerland) · 2024Article
- Microfluidic design for in-vitro liver zonation-a numerical analysis using COMSOL Multiphysics.Medical & biological engineering & computing · 2024Article
- Recent advances in liver-on-chips: Design, fabrication, and applications.Smart medicine · 2023Review
- Functional Evaluation of 3D Liver Models Labeled with Polysaccharide Functionalized Magnetic Nanoparticles.Materials (Basel, Switzerland) · 2022Article
- Physiologically relevant microsystems to study viral infection in the human liver.Frontiers in microbiology · 2022Review
- Chloroquine diphosphate suppresses liver cancer via inducing apoptosis in Wistar rats using interventional therapy.Oncology letters · 2021Article
- Engineering liver microtissues for disease modeling and regenerative medicine.Advanced functional materials · 2020Article
- iPSC-Derived Liver Organoids: A Journey from Drug Screening, to Disease Modeling, Arriving to Regenerative Medicine.International journal of molecular sciences · 2020Review
- Development of 3D Hepatic Constructs Within Polysaccharide-Based Scaffolds with Tunable Properties.International journal of molecular sciences · 2020Article
- Development of a tunable method to generate various three-dimensional microstructures by replenishing macromolecules such as extracellular matrix components and polysaccharides.Scientific reports · 2020Article
- Engineered Liver-on-a-Chip Platform to Mimic Liver Functions and Its Biomedical Applications: A Review.Micromachines · 2019Review
- Cytokines in adipose-derived mesenchymal stem cells promote the healing of liver disease.World journal of stem cells · 2018Review
- Advances in Engineered Human Liver Platforms for Drug Metabolism Studies.Drug metabolism and disposition: the biological fate of chemicals · 2018Article
- High-glucose 3D INS-1 cell model combined with a microfluidic circular concentration gradient generator for high throughput screening of drugs against type 2 diabetes.RSC advances · 2018Article
- Bioengineered Liver Models for Drug Testing and Cell Differentiation Studies.Cellular and molecular gastroenterology and hepatology · 2018Review
- The Microwell-mesh: A high-throughput 3D prostate cancer spheroid and drug-testing platform.Scientific reports · 2018Article
- Engineered Liver Platforms for Different Phases of Drug Development.Trends in biotechnology · 2017Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In drug discovery, it is very important to evaluate liver cells within an organism. Compared to 2D culture methods, the development of 3D culture techniques for liver cells has been successful in maintaining long-term liver functionality with the formation of a hepatic-specific structure. The key to performing drug testing is the establishment of a stable in vitro evaluation system. In this article, we report a Tapered Stencil for Cluster Culture (TASCL) device developed to create liver spheroids in vitro. The TASCL device will be applied as a toxicity evaluation system for drug discovery. The TASCL device was created with an overall size of 10 mm × 10 mm, containing 400 microwells with a top aperture (500 µm × 500 µm) and a bottom aperture (300 µm diameter circular) per microwell. We evaluated the formation, recovery, and size of HepG2 spheroids in the TASCL device. The formation and recovery were both nearly 100%, and the size of the HepG2 spheroids increased with an increase in the initial cell seeding density. There were no significant differences in the sizes of the spheroids among the microwells. In addition, the HepG2 spheroids obtained using the TASCL device were alive and produced albumin. The morphology of the HepG2 spheroids was investigated using FE-SEM. The spheroids in the microwells exhibited perfectly spherical aggregation. In this report, by adjusting the size of the microwells of the TASCL device, uniform HepG2 spheroids were created, and the device facilitated more precise measurements of the liver function per HepG2 spheroid. Our TASCL device will be useful for application as a toxicity evaluation system for drug testing.
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