Evidence map›Paper›PMID 26858904›Full record

ArticleCell medicine2015

Choice of Feeders Is Important When First Establishing iPSCs Derived From Primarily Cultured Human Deciduous Tooth Dental Pulp Cells.

Issei Saitoh, Emi Inada, Yoko Iwase, Hirofumi Noguchi, Tomoya Murakami, Miki Soda, Naoko Kubota, Hiroko Hasegawa, Eri Akasaka, Yuko Matsumoto and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Issei SaitohDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Gakkocho-dori, Chuo-ku, Niigata , Japan.
Emi Inada† Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Yoko IwaseDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Gakkocho-dori, Chuo-ku, Niigata , Japan.
Hirofumi Noguchi‡ Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus , Nishiharatyoaza, Uehara, Okinawa , Japan.
Tomoya MurakamiDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Gakkocho-dori, Chuo-ku, Niigata , Japan.
Miki SodaDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Gakkocho-dori, Chuo-ku, Niigata , Japan.
Naoko Kubota† Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Hiroko Hasegawa† Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Eri Akasaka† Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Yuko Matsumoto† Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Kyoko Oka§ Section of Pediatric Dentistry Department of Oral Growth and Development Fukuoka Dental College , Sawara-ku, Tamura Fukuoka-shi, Fukuoka , Japan.
Youichi Yamasaki† Department of Pediatric Dentistry, Kagoshima University Graduate School of Medical and Dental Sciences , Sakuragaoka, Kagoshima , Japan.
Haruaki HayasakiDivision of Pediatric Dentistry, Graduate School of Medical and Dental Science, Niigata University , Gakkocho-dori, Chuo-ku, Niigata , Japan.
Masahiro Sato¶ Section of Gene Expression Regulation, Frontier Science Research Center, Kagoshima University , Sakuragaoka, Kagoshima , Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Feeder cells are generally required to maintain embryonic stem cells (ESCs)/induced pluripotent stem cells (iPSCs). Mouse embryonic fibroblasts (MEFs) isolated from fetuses and STO mouse stromal cell line are the most widely used feeder cells. The aim of this study was to determine which cells are suitable for establishing iPSCs from human deciduous tooth dental pulp cells (HDDPCs). Primary cultures of HDDPCs were cotransfected with three plasmids containing human OCT3/4, SOX2/KLF4, or LMYC/LIN28 and pmaxGFP by using a novel electroporation method, and then cultured in an ESC qualified medium for 15 days. Emerging colonies were reseeded onto mitomycin C-treated MEFs or STO cells. The colonies were serially passaged for up to 26 passages. During this period, colony morphology was assessed to determine whether cells exhibited ESC-like morphology and alkaline phosphatase activity to evaluate the state of cellular reprogramming. HDDPCs maintained on MEFs were successfully reprogrammed into iPSCs, whereas those maintained on STO cells were not. Once established, the iPSCs were maintained on STO cells without loss of pluripotency. Our results indicate that MEFs are better feeder cells than STO cells for establishing iPSCs. Feeder choice is a key factor enabling efficient generation of iPSCs.

Indexed as

Deciduous toothDental pulpFeeder cellInduced pluripotent stem cells (iPSCs)Mouse embryonic fibroblasts (MEFs)STO cells

Identifiers

PMID26858904
PMCPMC4733907

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.