Evidence map›Paper›PMID 26858869›Full record

ArticleCell medicine2013

Use of Mesenchymal Stem Cell-Conditioned Medium to Activate Islets in Preservation Solution.

Naoya Kasahara, Takumi Teratani, Junshi Doi, Yuki Iijima, Masashi Maeda, Shinji Uemoto, Yasuhiro Fujimoto, Naohiro Sata, Yoshikazu Yasuda, Eiji Kobayashi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Improved Preservation of Rat Small Intestine Transplantation Graft by Introduction of Mesenchymal Stem Cell-Secreted Fractions.Transplant international : official journal of the European Society for Organ Transplantation · 2024
    Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Naoya KasaharaDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsukeshi, Tochigi, Japan; †Department of Surgery, Jichi Medical University, Shimotsukeshi, Tochigi, Japan.
Takumi TerataniDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
Junshi DoiDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
Yuki IijimaDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
Masashi MaedaDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
Shinji Uemoto‡ Division of Hepato-Pancreato-Biliary Surgery and Transplantation, Department of Surgery, Kyoto University Graduate School of Medicine , Syougoin, Sakyoku, Kyotoshi, Kyoto , Japan.
Yasuhiro FujimotoDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University, Shimotsukeshi, Tochigi, Japan; ‡Division of Hepato-Pancreato-Biliary Surgery and Transplantation, Department of Surgery, Kyoto University Graduate School of Medicine, Syougoin, Sakyoku, Kyotoshi, Kyoto, Japan.
Naohiro Sata† Department of Surgery, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
Yoshikazu Yasuda† Department of Surgery, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.
Eiji KobayashiDivision of Development of Advanced Therapy, Center for Development of Advanced Medical Technology, Jichi Medical University , Shimotsukeshi, Tochigi , Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pancreatic islet transplantation has received widespread attention as a promising treatment for type 1 diabetes. However, islets for transplantation are subject to damage from a number of sources, including ischemic injury during removal and delivery of the donor pancreas, enzymatic digestion during islet isolation, and reperfusion injury after transplantation in the recipient. Here we found that protein fractions secreted by mesenchymal stem cells (MSCs) were capable of activating preserved islets. A conditioned medium from the supernatant obtained by culturing adipose tissue MSCs (derived from wild-type Lewis rats) was prepared for 2 days in serum-free medium. Luc-Tg rat islets to which an organ preservation solution was added were then incubated at 4°C with fractions of various molecular weights prepared from the conditioned medium. Under the treatment with some of the fractions, by 4 days the relative luminescence intensities (representative of the ATP levels of the cold-preserved islets) had increased to over 150% of their initial values. Our novel system may be able to restore isolated islets to the condition they were in before transport, culture, and transplantation.

Indexed as

Living imageMesenchymal stem cells (MSCs)Preservation of isletsTransgenic rat

Identifiers

PMID26858869
PMCPMC4733859

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.