ArticleFrontiers in plant science2015
In vitro Biochemical Characterization of All Barley Endosperm Starch Synthases.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 55 citations in OpenAlex.
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- The influence of systematic heat and drought applications at defined growth stages on malting barley starch properties.Journal of the science of food and agriculture · 2025Article
- Kinetic and Structural Studies of the PlastidialACS omega · 2024Article
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
- Initiation of B-type starch granules in wheat endosperm requires the plastidial α-glucan phosphorylase PHS1.The Plant cell · 2023Article
- Site-Directed Mutations at Phosphorylation Sites inPlants (Basel, Switzerland) · 2023Article
- A model for the reproduction of amylopectin cluster by coordinated actions of starch branching enzyme isoforms.Plant molecular biology · 2023Review
- Loss of PROTEIN TARGETING TO STARCH 2 has variable effects on starch synthesis across organs and species.Journal of experimental botany · 2022Article
- Dynamic Change in Starch Biosynthetic Enzymes Complexes during Grain-Filling Stages in BEIIb Active and Deficient Rice.International journal of molecular sciences · 2022Article
- On the cluster structure of amylopectin.Plant molecular biology · 2022Review
- Starch synthases SSIIa and GBSSI control starch structure but do not determine starch granule morphology in the absence of SSIIIa and SSIVb.Plant molecular biology · 2022Article
- Molecular Functions and Pathways of Plastidial Starch Phosphorylase (PHO1) in Starch Metabolism: Current and Future Perspectives.International journal of molecular sciences · 2021Review
- Starch biosynthesis in cereal endosperms: An updated review over the last decade.Plant communications · 2021Review
- Integrated Transcriptomic and Bioinformatics Analyses Reveal the Molecular Mechanisms for the Differences in Seed Oil and Starch Content BetweenFrontiers in plant science · 2021Article
- A review of starch biosynthesis in cereal crops and its potential breeding applications in rice (PeerJ · 2021Article
- Genes That Mediate Starch Metabolism in Developing and Germinated Barley Grain.Frontiers in plant science · 2021Article
- A Review of Starch Biosynthesis in Relation to the Building Block-Backbone Model.International journal of molecular sciences · 2020Review
- Theoretical and experimental approaches to understand the biosynthesis of starch granules in a physiological context.Photosynthesis research · 2020Article
- Expression of starch-binding factor CBM20 in barley plastids controls the number of starch granules and the level of CO2 fixation.Journal of experimental botany · 2020Article
- Amylopectin Chain Length Dynamics and Activity Signatures of Key Carbon Metabolic Enzymes Highlight Early Maturation as Culprit for Yield Reduction of Barley Endosperm Starch after Heat Stress.Plant & cell physiology · 2019Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Starch is the main storage polysaccharide in cereals and the major source of calories in the human diet. It is synthesized by a panel of enzymes including five classes of starch synthases (SSs). While the overall starch synthase (SS) reaction is known, the functional differences between the five SS classes are poorly understood. Much of our knowledge comes from analyzing mutant plants with altered SS activities, but the resulting data are often difficult to interpret as a result of pleitropic effects, competition between enzymes, overlaps in enzyme activity and disruption of multi-enzyme complexes. Here we provide a detailed biochemical study of the activity of all five classes of SSs in barley endosperm. Each enzyme was produced recombinantly in E. coli and the properties and modes of action in vitro were studied in isolation from other SSs and other substrate modifying activities. Our results define the mode of action of each SS class in unprecedented detail; we analyze their substrate selection, temperature dependence and stability, substrate affinity and temporal abundance during barley development. Our results are at variance with some generally accepted ideas about starch biosynthesis and might lead to the reinterpretation of results obtained in planta. In particular, they indicate that granule bound SS is capable of processive action even in the absence of a starch matrix, that SSI has no elongation limit, and that SSIV, believed to be critical for the initiation of starch granules, has maltoligosaccharides and not polysaccharides as its preferred substrates.
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