ArticleSaudi journal of biological sciences2016
Measurement of filter paper activities of cellulase with microplate-based assay.
Article in Saudi journal of biological sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Screening and ARTP mutagenesis-based breeding of high-cellulase-producing strains.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Kombucha-Derived Cellulose Non-wovens: Growth Optimization, Mechanics, and Recycling.bioRxiv : the preprint server for biology · 2026Article
- Saccharification and co-fermentation of lignocellulosic biomass by a cockroach-gut bacterial symbiont and yeast cocktail for bioethanol production.BMC biotechnology · 2024Article
- Enhanced production of bioethanol through supercritical carbon dioxide-mediated pretreatment and saccharification of dewaxed bagasse.Scientific reports · 2024Article
- Diversity and Screening of Cellulolytic Microorganisms from Mangrove Forests, Natural Parks, Paddy Field, and Sugarcane Plantation in Panay Island, Philippines.International journal of microbiology · 2024Article
- Solid-state fermentation of Saba banana peel for pigment production by Monascus purpureus.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Evaluation of Preparation and Detoxification of Hemicellulose Hydrolysate for Improved Xylitol Production from Quinoa Straw.International journal of molecular sciences · 2022Article
- A processive GH9 family endoglucanase of Bacillus licheniformis and the role of its carbohydrate-binding domain.Applied microbiology and biotechnology · 2022Article
- Synergistic hydrolysis of filter paper by recombinant cellulase cocktails leveraging a key cellobiase, Cba2, ofFrontiers in bioengineering and biotechnology · 2022Article
- Integration of Solid State and Submerged Fermentations for the Valorization of Organic Municipal Solid Waste.Journal of fungi (Basel, Switzerland) · 2021Article
- Dynamics of Fusarium Mycotoxins and Lytic Enzymes during Pea Plants' Infection.International journal of molecular sciences · 2021Article
- Induction ofPathogens (Basel, Switzerland) · 2020Article
- Enhancement the Cellulase Activity Induced by Endophytic Bacteria Using Calcium Nanoparticles.Current microbiology · 2019Article
- Lignocellulose utilization and bacterial communities of millet straw based mushroom (Agaricus bisporus) production.Scientific reports · 2019Article
- Thermostable Cellulases from the YeastEnzyme research · 2019Article
- Addition of aluminum oxide microparticles toEngineering in life sciences · 2018Article
- Improvement of Animal Feed Additives of Ginkgo Leaves through Solid-state Fermentation usingInternational journal of biological sciences · 2018Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is always a challenge to determine the total cellulase activity efficiently without reducing accuracy. The most common total cellulase activity assay is the filter paper assay (FPA) established by the International Union of Pure and Applied Chemistry (IUPAC). A new procedure to measure the FPA with microplate-based assay was studied in this work, which followed the main idea of IUPAC to dilute cellulase preparation to get fixed glucose release. FPAs of six cellulase preparations were determined with the microplate-based assay. It is shown that FPAs of cellulase Youtell, RCconc, R-10, Lerkam, Yishui and Sinopharm were 67.9, 46.0, 46.1, 27.4, 7.6 and 8.0 IU/ml respectively. There was no significant difference at the 95% confidence level between the FPA determined with IUPAC and the microplate-based assay. It could be concluded that the FPA could be determined by the microplate-based assay with the same accuracy and much more efficiency compared with that by IUPAC.
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