Evidence map›Paper›PMID 26858453›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.

Joanna Kowal, Guillaume Arras, Marina Colombo, Mabel Jouve, Jakob Paul Morath, Bjarke Primdal-Bengtson, Florent Dingli, Damarys Loew, Mercedes Tkach, Clotilde Théry

Registry-linked trialOpen access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT05491395 (Randomized Phase III Clinical Trial of Hypofractionated Radiotherapy in Breast Cancer Patients With Immediate Prosthetic Reconstruction), which is not on this map. Cited by 2,142 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2,142citing papers in PubMed, 3 pooled it
150.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT05491395 narecruitingnot on this mapstarted 2022, after this paper: background citation

Randomized Phase III Clinical Trial of Hypofractionated Radiotherapy in Breast Cancer Patients With Immediate Prosthetic Reconstruction: PROMART Trial

TypeinterventionalSponsorBarretos Cancer HospitalRan2022 to 2029Enrolled120ConditionsBreast CancerArmsHypofractionation
3 · Its place in the literature

Who cites it

2,142 citing papers in PubMed, 3 syntheses or guidelines pooled it, 3,355 citations in OpenAlex.

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  13. A Novel Reporter Mouse for Astrocyte-Derived Extracellular Vesicles Reveals Enrichment of Cargo at Neuronal Mitochondria.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
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2,082 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Joanna KowalInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France;
Guillaume ArrasInstitut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de masse Protéomique, 75248 Paris, France.
Marina ColomboInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France;
Mabel JouveInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France;
Jakob Paul MorathInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France;
Bjarke Primdal-BengtsonInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France;
Florent DingliInstitut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de masse Protéomique, 75248 Paris, France.
Damarys LoewInstitut Curie, PSL Research University, Centre de Recherche, Laboratoire de Spectrométrie de masse Protéomique, 75248 Paris, France.ORCID http://orcid.org/0000-0002-9111-8842
Mercedes TkachInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France;
Clotilde ThéryInstitut Curie, PSL Research University, INSERM U932, Department "Immunité et Cancer", 75248 Paris, France; clotilde.thery@curie.fr.
Inserm · FRUniversité Paris Sciences et Lettres · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extracellular vesicles (EVs) have become the focus of rising interest because of their numerous functions in physiology and pathology. Cells release heterogeneous vesicles of different sizes and intracellular origins, including small EVs formed inside endosomal compartments (i.e., exosomes) and EVs of various sizes budding from the plasma membrane. Specific markers for the analysis and isolation of different EV populations are missing, imposing important limitations to understanding EV functions. Here, EVs from human dendritic cells were first separated by their sedimentation speed, and then either by their behavior upon upward floatation into iodixanol gradients or by immuno-isolation. Extensive quantitative proteomic analysis allowing comparison of the isolated populations showed that several classically used exosome markers, like major histocompatibility complex, flotillin, and heat-shock 70-kDa proteins, are similarly present in all EVs. We identified proteins specifically enriched in small EVs, and define a set of five protein categories displaying different relative abundance in distinct EV populations. We demonstrate the presence of exosomal and nonexosomal subpopulations within small EVs, and propose their differential separation by immuno-isolation using either CD63, CD81, or CD9. Our work thus provides guidelines to define subtypes of EVs for future functional studies.

Indexed as

ProteomicsAntigens, CDBiomarkersCell-Derived MicroparticlesHSP70 Heat-Shock ProteinsHumansMembrane ProteinsAntigens, CDBiomarkersflotillinsHSP70 Heat-Shock ProteinsMembrane Proteinsdendritic cellsectosomesexosomesextracellular vesiclesmicrovesicles

Identifiers

PMID26858453
PMCPMC4776515
OpenAlexW2259481044

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.