ArticleProceedings of the National Academy of Sciences of the United States of America2016
Variable priming of a docked synaptic vesicle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Vesicle3D: An Integrative Platform for 3D Segmentation and Analysis of Vesicles in Cryo-electron Tomograms.Neuroscience bulletin · 2026Article
- High-Pressure Freezing EM Tomography of Entire Ribbon Synapses in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Enhanced cycling of presynaptic vesicles during long-term potentiation in rat hippocampus.The Journal of physiology · 2025Review
- A hemifused complex is the hub in a network of pathways to membrane fusion.Biophysical journal · 2023Article
- Cryo-EM tomography and automatic segmentation delineate modular structures in the postsynaptic density.Frontiers in synaptic neuroscience · 2023Article
- The premetazoan ancestry of the synaptic toolkit and appearance of first neurons.Essays in biochemistry · 2022Article
- Presynaptic Rac1 controls synaptic strength through the regulation of synaptic vesicle priming.eLife · 2022Article
- Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX.Science advances · 2022Article
- A two-subpopulation model that reflects heterogeneity of large dense core vesicles in exocytosis.Cell cycle (Georgetown, Tex.) · 2022Article
- Microphysiological Modeling of the Structure and Function of Neuromuscular Transmitter Release Sites.Frontiers in synaptic neuroscience · 2022Review
- Shortened tethering filaments stabilize presynaptic vesicles in support of elevated release probability during LTP in rat hippocampus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Dynamics of Neuromuscular Transmission Reproduced by Calcium-Dependent and Reversible Serial Transitions in the Vesicle Fusion Complex.Frontiers in synaptic neuroscience · 2021Article
- The Mechanical Basis of Memory - the MeshCODE Theory.Frontiers in molecular neuroscience · 2021Article
- Hypothesis Relating the Structure, Biochemistry and Function of Active Zone Material Macromolecules at a Neuromuscular Junction.Frontiers in synaptic neuroscience · 2021Article
- Continuum Models of Membrane Fusion: Evolution of the Theory.International journal of molecular sciences · 2020Review
- Synaptic Vesicles Having Large Contact Areas with the Presynaptic Membrane are Preferentially Hemifused at Active Zones of Frog Neuromuscular Junctions Fixed during Synaptic Activity.International journal of molecular sciences · 2019Review
- Nanomachinery Organizing Release at Neuronal and Ribbon Synapses.International journal of molecular sciences · 2019Review
- Differentiation and Characterization of Excitatory and Inhibitory Synapses by Cryo-electron Tomography and Correlative Microscopy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Impact of spatiotemporal calcium dynamics within presynaptic active zones on synaptic delay at the frog neuromuscular junction.Journal of neurophysiology · 2018Article
- Distribution of vesicle pools in cerebellar parallel fibre terminals after depression of ectopic transmission.PloS one · 2018Article
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4 authors.
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Abstract
The priming of a docked synaptic vesicle determines the probability of its membrane (VM) fusing with the presynaptic membrane (PM) when a nerve impulse arrives. To gain insight into the nature of priming, we searched by electron tomography for structural relationships correlated with fusion probability at active zones of axon terminals at frog neuromuscular junctions. For terminals fixed at rest, the contact area between the VM of docked vesicles and PM varied >10-fold with a normal distribution. There was no merging of the membranes. For terminals fixed during repetitive evoked synaptic transmission, the normal distribution of contact areas was shifted to the left, due in part to a decreased number of large contact areas, and there was a subpopulation of large contact areas where the membranes were hemifused, an intermediate preceding complete fusion. Thus, fusion probability of a docked vesicle is related to the extent of its VM-PM contact area. For terminals fixed 1 h after activity, the distribution of contact areas recovered to that at rest, indicating the extent of a VM-PM contact area is dynamic and in equilibrium. The extent of VM-PM contact areas in resting terminals correlated with eccentricity in vesicle shape caused by force toward the PM and with shortness of active zone material macromolecules linking vesicles to PM components, some thought to include Ca(2+) channels. We propose that priming is a variable continuum of events imposing variable fusion probability on each vesicle and is regulated by force-generating shortening of active zone material macromolecules in dynamic equilibrium.
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