ArticleProceedings of the National Academy of Sciences of the United States of America2016
Engineering super mycovirus donor strains of chestnut blight fungus by systematic disruption of multilocus vic genes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
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- Regulated cell death in fungi from a comparative immunology perspective.Cell death and differentiation · 2026Review
- The Role of Phyllosphere Microbes and Viruses in Biocontrol of Pathogenic Fungi.Microbial biotechnology · 2025Review
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- Plants interfere with non-self recognition of a phytopathogenic fungus via proline accumulation to facilitate mycovirus transmission.Nature communications · 2024Article
- Review
- Viruses of plant-pathogenic fungi: a promising biocontrol strategy for Sclerotinia sclerotiorum.Archives of microbiology · 2023Review
- Emergence of the fungal immune system.iScience · 2023Review
- Review
- Physiological variations in hypovirus-infected wild and model long-term laboratory strains ofFrontiers in microbiology · 2023Article
- Metatranscriptome-based strategy reveals the existence of novel mycoviruses in the plant pathogenic fungusFrontiers in microbiology · 2023Article
- Article
- Fungal gasdermin-like proteins are controlled by proteolytic cleavage.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Two Pathogenic Fungi Isolated From Chalkbrood Samples and Honey Bee Viruses They Carried.Frontiers in microbiology · 2022Article
- Article
- In-Tree Behavior of Diverse Viruses Harbored in the Chestnut Blight Fungus,Journal of virology · 2021Article
- Divergent RNA viruses inVirus evolution · 2021Article
- Deep learning approaches for natural product discovery from plant endophytic microbiomes.Environmental microbiome · 2021Review
- Structures of Pathological and Functional Amyloids and Prions, a Solid-State NMR Perspective.Frontiers in molecular neuroscience · 2021Review
- Article
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2 authors.
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Abstract
Transmission of mycoviruses that attenuate virulence (hypovirulence) of pathogenic fungi is restricted by allorecognition systems operating in their fungal hosts. We report the use of systematic molecular gene disruption and classical genetics for engineering fungal hosts with superior virus transmission capabilities. Four of five diallelic virus-restricting allorecognition [vegetative incompatibility (vic)] loci were disrupted in the chestnut blight fungus Cryphonectria parasitica using an adapted Cre-loxP recombination system that allowed excision and recycling of selectable marker genes (SMGs). SMG-free, quadruple vic mutant strains representing both allelic backgrounds of the remaining vic locus were then produced through mating. In combination, these super donor strains were able to transmit hypoviruses to strains that were heteroallelic at one or all of the virus-restricting vic loci. These results demonstrate the feasibility of modulating allorecognition to engineer pathogenic fungi for more efficient transmission of virulence-attenuating mycoviruses and enhanced biological control potential.
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