ArticleProceedings of the National Academy of Sciences of the United States of America2016
Structure-function analysis of myomaker domains required for myoblast fusion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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Who cites it
57 citing papers in PubMed, 83 citations in OpenAlex.
- Dosage-sensitive regulation of Cofilin-1 and LIMK signaling controls myoblast differentiation and fusion.Cellular and molecular life sciences : CMLS · 2026Article
- Species-specific functions of CSRP3 in satellite cell-driven muscle regeneration and relevance to porcine stem cell therapy.Cell regeneration (London, England) · 2026Article
- The Secretome of a Cachexia-Inducing Lung Tumor Impairs Mitochondrial Function and Skeletal Muscle Differentiation.Cancers · 2026Article
- Article
- Dynamic expression and differential requirement of the myocyte fusogen Myomixer during distinct myogenic episodes in the zebrafish.Biology open · 2025Article
- Impaired myogenesis in limb girdle muscular dystrophy type 2B.Scientific reports · 2025Article
- VEGF-B-mediated myofiber types involved in high-fat diet-induced hyperglycemia through PKA-NFATs signaling pathway.Stem cell research & therapy · 2025Article
- Novel drug-inducible CRISPRa/i systems for rapid and reversible manipulation of gene transcription.Cellular and molecular life sciences : CMLS · 2025Article
- The miR-6240 target gene Igf2bp3 promotes myoblast fusion by enhancing myomaker mRNA stability.Cellular & molecular biology letters · 2024Article
- Ectopic expression of Myomaker and Myomixer in slow muscle cells induces slow muscle fusion and myofiber death.Journal of genetics and genomics = Yi chuan xue bao · 2024Article
- The Regulatory Role ofAnimals : an open access journal from MDPI · 2024Article
- Review
- Division-Independent Differentiation of Muscle Stem Cells During a Growth Stimulus.Stem cells (Dayton, Ohio) · 2024Article
- Molecular regulation of myocyte fusion.Current topics in developmental biology · 2024Review
- A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization.Biomolecules · 2023Review
- The Mouse CircGHR Regulates Proliferation, Differentiation and Apoptosis of Hepatocytes and Myoblasts.Genes · 2023Article
- Expression of Myomaker and Myomerger in myofibers causes muscle pathology.Skeletal muscle · 2023Article
- Myomaker and Myomixer Characterization in Gilthead Sea Bream under Different Myogenesis Conditions.International journal of molecular sciences · 2022Article
- Early tension regulation coupled to surface myomerger is necessary for the primary fusion of C2C12 myoblasts.Frontiers in physiology · 2022Article
- Evolution of a chordate-specific mechanism for myoblast fusion.Science advances · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
During skeletal muscle development, myoblasts fuse to form multinucleated myofibers. Myomaker [Transmembrane protein 8c (TMEM8c)] is a muscle-specific protein that is essential for myoblast fusion and sufficient to promote fusion of fibroblasts with muscle cells; however, the structure and biochemical properties of this membrane protein have not been explored. Here, we used CRISPR/Cas9 mutagenesis to disrupt myomaker expression in the C2C12 muscle cell line, which resulted in complete blockade to fusion. To define the functional domains of myomaker required to direct fusion, we established a heterologous cell-cell fusion system, in which fibroblasts expressing mutant versions of myomaker were mixed with WT myoblasts. Our data indicate that the majority of myomaker is embedded in the plasma membrane with seven membrane-spanning regions and a required intracellular C-terminal tail. We show that myomaker function is conserved in other mammalian orthologs; however, related family members (TMEM8a and TMEM8b) do not exhibit fusogenic activity. These findings represent an important step toward deciphering the cellular components and mechanisms that control myoblast fusion and muscle formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.