ArticleProceedings of the National Academy of Sciences of the United States of America2016
Deterministically patterned biomimetic human iPSC-derived hepatic model via rapid 3D bioprinting.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 373 papers.
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Who cites it
373 citing papers in PubMed.
- 3D bioprinting of tissues and organs for systemic diseases and localized injuries.Military Medical Research · 2026Review
- Photo-Responsive Functional Hydrogels: Mechanism, Design, and Application.Chemistry, an Asian journal · 2026Review
- Engineering heterogeneous tissues and organs via multi-material bioprinting: Advances, challenges, and opportunities.Acta biomaterialia · 2026Review
- Biomedical Materials and Fabrication Methods for Construction of In Vitro Neurovascular Unit Models.Materials (Basel, Switzerland) · 2026Review
- Advances in vascularized organoids.Chinese medical journal · 2026Review
- Biofabrication for spatial control of multiscale biological crosstalk in tissue models.npj biomedical innovations · 2026Review
- Article
- Digital light processing bioprinting: bioink innovations and applications in tissue and organ regeneration.Journal of biological engineering · 2026Review
- Liver-on-Chip: An Analysis of Liver Cell Types, Seeding Parameters, and Liver Function Assays.Micromachines · 2026Review
- 3D Printing with Marine Gelatin: A Cross-Sector Review of Biomedical, Food, and Health Uses.Marine drugs · 2026Review
- A degradable PEGDA-dopamine hydrogel with ROS scavenging capacity supports flexible design for nerve repair.Materials today. Bio · 2026Article
- Biomimetic villi-crypt scaffold-on-chip with tunable mechanical properties for intestinal epithelium modeling.Materials today. Bio · 2026Article
- Improving hepatic metabolic function with 3D iPSC-derived human hepatocytes.Cell biology and toxicology · 2026Article
- Mechanobiology in Stem Cell-Based Bioprinting.Cell proliferation · 2026Review
- Microengineering the Liver: Strategies for Constructing Functional Liver-on-a-Chip Devices.Exploration (Beijing, China) · 2026Review
- Micropatterned DNA Hydrogels for Spatiotemporal Programming of Chemical Reaction Networks.ACS nano · 2026Article
- 3D biofabricated in vitro models as new approach methodologies for animal alternatives.npj biomedical innovations · 2026Review
- Acoustic Bioprinting: A Glimpse Into an Emerging Field.Small methods · 2026Review
- Combination of 3D Micro Contact Printing and Scraping Technique for Site Selective Cocultivation Across a Porous Micro Structured Scaffold.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Article
313 more citing papers are in PubMed but not listed here.
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14 authors.
Funding
Abstract
The functional maturation and preservation of hepatic cells derived from human induced pluripotent stem cells (hiPSCs) are essential to personalized in vitro drug screening and disease study. Major liver functions are tightly linked to the 3D assembly of hepatocytes, with the supporting cell types from both endodermal and mesodermal origins in a hexagonal lobule unit. Although there are many reports on functional 2D cell differentiation, few studies have demonstrated the in vitro maturation of hiPSC-derived hepatic progenitor cells (hiPSC-HPCs) in a 3D environment that depicts the physiologically relevant cell combination and microarchitecture. The application of rapid, digital 3D bioprinting to tissue engineering has allowed 3D patterning of multiple cell types in a predefined biomimetic manner. Here we present a 3D hydrogel-based triculture model that embeds hiPSC-HPCs with human umbilical vein endothelial cells and adipose-derived stem cells in a microscale hexagonal architecture. In comparison with 2D monolayer culture and a 3D HPC-only model, our 3D triculture model shows both phenotypic and functional enhancements in the hiPSC-HPCs over weeks of in vitro culture. Specifically, we find improved morphological organization, higher liver-specific gene expression levels, increased metabolic product secretion, and enhanced cytochrome P450 induction. The application of bioprinting technology in tissue engineering enables the development of a 3D biomimetic liver model that recapitulates the native liver module architecture and could be used for various applications such as early drug screening and disease modeling.
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