ArticlePlant physiology2016
A Signaling Cascade from miR444 to RDR1 in Rice Antiviral RNA Silencing Pathway.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 1 synthesis or guideline pooled it, 162 citations in OpenAlex.
- What Do We Know about Barley miRNAs?International journal of molecular sciences · 2022Pooled it
- Regulatory role of epigenetics in rice immunity against bacterial and fungal pathogens.Crop health · 2026Review
- RNA regulation in plants.Science China. Life sciences · 2026Review
- Genome-wide identification and characterization of the RNAi gene families in Brassica rapa L. highlighting their regulatory components and underlying functions involving crop improvement.BMC genomic data · 2026Article
- CsRDR1b and CsRDR1c genes in Cucumis sativus are crucial for plant defense against virus and oomycete co-infection.Scientific reports · 2026Article
- Roles of MADS-box transcription factors in plant responses to abiotic and biotic stresses.Plant communications · 2026Review
- Identification and analysis of microRNAs responsible for brown planthopper resistance in BPH14 and BPH15 pyramiding rice.BMC plant biology · 2026Article
- Metavirome Detection and Analysis of Viruses Present in Diseased Pumpkin in Shandong, China.Viruses · 2026Article
- Plant-derived, stress-responsive microRNA mimics reduce powdery mildew infections of barley.Frontiers in plant science · 2026Article
- Small but big player: the important role of microRNAs in rice.Frontiers in plant science · 2026Review
- A receptor-like kinase recognizes viral proteins at the trans-Golgi network/early endosome and inhibits infection in rice.Cell discovery · 2025Article
- Antiviral RNA interference in plants: Increasing complexity and integration with other biological processes.Plant communications · 2025Review
- miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.Molecular plant pathology · 2025Article
- Article
- An elite RNA-DEPENDENT RNA POLYMERASE3 allele enhances preharvest sprouting resistance in rice.Plant physiology · 2025Article
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Evolutionary Dynamics and Expression Divergence of thePlants (Basel, Switzerland) · 2025Article
- Review
- Crop antiviral defense: Past and future perspective.Science China. Life sciences · 2024Review
- Major components of RNAi gene families inHeliyon · 2024Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant RNA-DEPENDENT RNA POLYMERASE1 (RDR1) is a key component of the antiviral RNA-silencing pathway, contributing to the biogenesis of virus-derived small interfering RNAs. This enzyme also is responsible for producing virus-activated endogenous small interfering RNAs to stimulate the broad-spectrum antiviral activity through silencing host genes. The expression of RDR1 orthologs in various plants is usually induced by virus infection. However, the molecular mechanisms of activation of RDR1 expression in response to virus infection remain unknown. Here, we show that a monocot-specific microRNA, miR444, is a key factor in relaying the antiviral signaling from virus infection to OsRDR1 expression. The expression of miR444 is enhanced by infection with Rice stripe virus (RSV), and overexpression of miR444 improves rice (Oryza sativa) resistance against RSV infection accompanied by the up-regulation of OsRDR1 expression. We further show that three miR444 targets, the MIKC(C)-type MADS box proteins OsMADS23, OsMADS27a, and OsMADS57, form homodimers and heterodimers between them to repress the expression of OsRDR1 by directly binding to the CArG motifs of its promoter. Consequently, an increased level of miR444 diminishes the repressive roles of OsMADS23, OsMADS27a, and OsMADS57 on OsRDR1 transcription, thus activating the OsRDR1-dependent antiviral RNA-silencing pathway. We also show that overexpression of miR444-resistant OsMADS57 reduced OsRDR1 expression and rice resistance against RSV infection, and knockout of OsRDR1 reduced rice resistance against RSV infection. In conclusion, our results reveal a molecular cascade in the rice antiviral pathway in which miR444 and its MADS box targets directly control OsRDR1 transcription.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.