ArticleMolecular therapy : the journal of the American Society of Gene Therapy2016
Genetic Manipulation of Brown Fat Via Oral Administration of an Engineered Recombinant Adeno-associated Viral Serotype Vector.
Article in Molecular therapy : the journal of the American Society of Gene Therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.
- New Mediators in the Crosstalk between Different Adipose Tissues.International journal of molecular sciences · 2024Pooled it
- Article
- An efficient AAV vector system of Rec2 serotype for intravenous injection to study metabolism in brown adipocytes in vivo.Molecular metabolism · 2024Article
- AAV-Mediated Gene Delivery to Mouse Brown Adipose Tissue.Methods in molecular biology (Clifton, N.J.) · 2023Article
- LMO3 reprograms visceral adipocyte metabolism during obesity.Journal of molecular medicine (Berlin, Germany) · 2021Article
- Therapeutic Perspectives of Thermogenic Adipocytes in Obesity and Related Complications.International journal of molecular sciences · 2021Review
- Environmental activation of a hypothalamic BDNF-adipocyte IL-15 axis regulates adipose-natural killer cells.Brain, behavior, and immunity · 2021Article
- Visceral adipose tissue-directed FGF21 gene therapy improves metabolic and immune health in BTBR mice.Molecular therapy. Methods & clinical development · 2021Article
- Adipose Tissue: An Emerging Target for Adeno-associated Viral Vectors.Molecular therapy. Methods & clinical development · 2020Review
- Viral and Nonviral Transfer of Genetic Materials to Adipose Tissues: Toward a Gold Standard Approach.Diabetes · 2020Review
- Adeno-Associated Virus (AAV) Capsid Stability and Liposome Remodeling During Endo/Lysosomal pH Trafficking.Viruses · 2020Article
- Adipose PTEN regulates adult adipose tissue homeostasis and redistribution via a PTEN-leptin-sympathetic loop.Molecular metabolism · 2019Article
- Brown and Brite: The Fat Soldiers in the Anti-obesity Fight.Frontiers in physiology · 2019Review
- Targeting Visceral Fat by Intraperitoneal Delivery of Novel AAV Serotype Vector Restricting Off-Target Transduction in Liver.Molecular therapy. Methods & clinical development · 2017Article
- Improved gene delivery to adult mouse spinal cord through the use of engineered hybrid adeno-associated viral serotypes.Gene therapy · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Recombinant adeno-associated virus (rAAV) vectors are attractive vehicles for gene therapy. Gene delivery to the adipose tissue using naturally occurring AAV serotypes is less successful compared to liver and muscle. Here, we demonstrate that oral administration of an engineered serotype Rec2 led to preferential transduction of brown fat with absence of transduction in the gastrointestinal track. Among the six natural and engineered serotypes being compared, Rec2 was the most efficient serotype achieving high level transduction at a dose 1~2 orders lower than reported doses for systemic administration. Overexpressing vascular endothelial growth factor (VEGF) in brown fat via oral administration of Rec2-VEGF vector increased the brown fat mass and enhanced thermogenesis. In contrast, knockdown VEGF in brown fat of VEGF (loxP) mice via Rec2-Cre vector hampered cold response and decreased brown fat mass. Oral administration of Rec2 vector provides a novel tool to genetically manipulate brown fat for research and therapeutic applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.