ArticleScientific reports2016
Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 136 citations in OpenAlex.
- Constructing pyrG marker by CRISPR/Cas facilities the highly-efficient precise genome editing on industrial Aspergillus niger strain.Bioprocess and biosystems engineering · 2025Article
- Metabolic Engineering of Filamentous FungusJournal of agricultural and food chemistry · 2025Article
- The Ras small GTPase RSR1 regulates cellulase production in Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2023Article
- Mechanism of ZnBiotechnology for biofuels and bioproducts · 2023Article
- Intracellular Sugar Transporters Facilitate Cellulase Synthesis inBiomolecules · 2023Article
- Establishment, optimization, and application of genetic technology inFrontiers in microbiology · 2023Review
- Roles of PKAc1 and CRE1 in cellulose degradation, conidiation, and yellow pigment synthesis in Trichoderma reesei QM6a.Biotechnology letters · 2022Article
- An overview on current molecular tools for heterologous gene expression in Trichoderma.Fungal biology and biotechnology · 2021Review
- Article
- High-dose rapamycin exerts a temporary impact on T. reesei RUT-C30 through gene trFKBP12.Biotechnology for biofuels · 2021Article
- cAMP activates calcium signalling via phospholipase C to regulate cellulase production in the filamentous fungus Trichoderma reesei.Biotechnology for biofuels · 2021Article
- Engineering ofBiotechnology for biofuels · 2020Article
- The transcription factor ACE3 controls cellulase activities and lactose metabolism via two additional regulators in the fungusThe Journal of biological chemistry · 2019Article
- Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening.Microbial cell factories · 2019Article
- Enhanced cellulase production by decreasing intercellular pH through HBiotechnology for biofuels · 2019Article
- Enhanced cellulase production in Trichoderma reesei RUT C30 via constitution of minimal transcriptional activators.Microbial cell factories · 2018Article
- Primers and copper responsive promoter design and data of real-time RT-PCR assay in filamentous fungusData in brief · 2018Article
- The Promoter Toolbox for Recombinant Gene Expression inFrontiers in bioengineering and biotechnology · 2018Review
- Construction of enhanced transcriptional activators for improving cellulase production inBioresources and bioprocessing · 2018Article
- MnBiotechnology for biofuels · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Filamentous fungi play important roles in the production of plant cell-wall degrading enzymes. In recent years, homologous recombinant technologies have contributed significantly to improved enzymes production and system design of genetically manipulated strains. When introducing multiple gene deletions, we need a robust and convenient way to control selectable marker genes, especially when only a limited number of markers are available in filamentous fungi. Integration after transformation is predominantly nonhomologous in most fungi other than yeast. Fungal strains deficient in the non-homologous end-joining (NHEJ) pathway have limitations associated with gene function analyses despite they are excellent recipient strains for gene targets. We describe strategies and methods to address these challenges above and leverage the power of resilient NHEJ deficiency strains. We have established a foolproof light-inducible platform for one-step unmarked genetic modification in industrial eukaryotic microorganisms designated as 'LML 3.0', and an on-off control protocol of NHEJ pathway called 'OFN 1.0', using a synthetic light-switchable transactivation to control Cre recombinase-based excision and inversion. The methods provide a one-step strategy to sequentially modify genes without introducing selectable markers and NHEJ-deficiency. The strategies can be used to manipulate many biological processes in a wide range of eukaryotic cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.