ArticleNucleic acids research1989
Characterization of the transcriptional potency of sub-elements of the UAS of the yeast PGK gene in a PGK mini-promoter.
Article in Nucleic acids research, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Homotypic cooperativity and collective binding are determinants of bHLH specificity and function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Glucose signaling in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 2006Review
- APOBEC-1 dependent cytidine to uridine editing of apolipoprotein B RNA in yeast.Nucleic acids research · 2000Article
- Multiple domains of repressor activator protein 1 contribute to facilitated binding of glycolysis regulatory protein 1.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The role of Gcr1p in the transcriptional activation of glycolytic genes in yeast Saccharomyces cerevisiae.Genetics · 1997Article
- Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p).Molecular and cellular biology · 1996Article
- The multifunctional transcription factors Abf1p, Rap1p and Reb1p are required for full transcriptional activation of the chromosomal PGK gene in Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1996Article
- Detection of antagonistic cellular regulatory functions by the gene-gene interference method in yeast.Current genetics · 1996Article
- Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae).Current genetics · 1995Review
- The yeast protein Gcr1p binds to the PGK UAS and contributes to the activation of transcription of the PGK gene.Molecular & general genetics : MGG · 1994Article
- The yeast co-activator GAL11 positively influences transcription of the phosphoglycerate kinase gene, but only when RAP1 is bound to its upstream activation sequence.Molecular & general genetics : MGG · 1994Article
- Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast.Molecular & general genetics : MGG · 1993Article
- Downstream activating sequence within the coding region of a yeast gene: specific binding in vitro of RAP1 protein.Molecular & general genetics : MGG · 1992Article
- A phylogenetic analysis based on the gene encoding phosphoglycerate kinase.Journal of molecular evolution · 1992Article
- Dissection of a carboxy-terminal region of the yeast regulatory protein RAP1 with effects on both transcriptional activation and silencing.Molecular and cellular biology · 1992Article
- Multifunctional DNA-binding proteins in yeast.Gene expression · 1992Review
- RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene.Molecular and cellular biology · 1991Article
- Phosphorylation influences the binding of the yeast RAP1 protein to the upstream activating sequence of the PGK gene.Nucleic acids research · 1990Article
- ARS binding factor 1 binds adjacent to RAP1 at the UASs of the yeast glycolytic genes PGK and PYK1.Nucleic acids research · 1990Article
- Characterisation of the DNA binding domain of the yeast RAP1 protein.Nucleic acids research · 1990Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The upstream activator (UAS) of the yeast PGK gene comprises three different sequence elements. These are 1) a region of strong protein binding called the YFP, 2) three repeats of the motif CTTCC and 3) an essential activator core (AC) sequence that binds the protein RAP1. To assess the function of each of these elements in transcriptional activation we have inserted them individually and in various combinations into a PGK mini-promoter. This comprises only the transcription initiation elements from the PGK promoter and is inactive in the absence of activator sequences. None of the individual sequence elements was capable of activating the mini-promoter. However either the YFP or the CTTCC boxes in conjunction with the AC box resulted in efficient expression. Transcription levels were not however as high as when all three elements were inserted. These data suggest that the efficiency of PGK transcription depends upon the interactions between three different sequences. Furthermore while RAP1 per se is not a transcriptional activator it can associate promiscuously with other factors to create a functional transcription complex.
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