ArticleNucleic acids research2016
Expression of multiple Bacillus subtilis genes is controlled by decay of slrA mRNA from Rho-dependent 3' ends.
Article in Nucleic acids research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Comprehensive mapping of transcription terminator Rho utilization (Rut) sites across the Bacillus subtilis genome.Nucleic acids research · 2025Article
- Termination factor Rho mediates transcriptional reprogramming of Bacillus subtilis stationary phase.PLoS genetics · 2023Article
- The potential of cold-shock promoters for the expression of recombinant proteins in microbes and mammalian cells.Journal, genetic engineering & biotechnology · 2022Review
- Comprehensive transcription terminator atlas for Bacillus subtilis.Nature microbiology · 2022Article
- Identification of Genes Required for Swarming Motility inJournal of bacteriology · 2022Article
- Article
- Discovery and initial characterization of YloC, a novel endoribonuclease inRNA (New York, N.Y.) · 2022Article
- Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli.Communications biology · 2021Article
- Polynucleotide phosphorylase and RNA helicase CshA cooperate inRNA biology · 2021Article
- Mastering the control of the Rho transcription factor for biotechnological applications.Applied microbiology and biotechnology · 2021Review
- Bacterial ribonucleases and their roles in RNA metabolism.Critical reviews in biochemistry and molecular biology · 2019Review
- Transcription termination factor Rho and microbial phenotypic heterogeneity.Current genetics · 2018Review
- AU-Rich Long 3' Untranslated Region Regulates Gene Expression in Bacteria.Frontiers in microbiology · 2018Article
- Termination factor Rho: From the control of pervasive transcription to cell fate determination in Bacillus subtilis.PLoS genetics · 2017Article
- Regulatory 3' Untranslated Regions of Bacterial mRNAs.Frontiers in microbiology · 2017Review
- Learning from the Leaders: Gene Regulation by the Transcription Termination Factor Rho.Trends in biochemical sciences · 2016Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Timely turnover of RNA is an important element in the control of bacterial gene expression, but relatively few specific targets of RNA turnover regulation are known. Deletion of the Bacillus subtilis pnpA gene, encoding the major 3' exonuclease turnover enzyme, polynucleotide phosphorylase (PNPase), was shown previously to cause a motility defect correlated with a reduced level of the 32-gene fla/che flagellar biosynthesis operon transcript.fla/che operon transcript abundance has been shown to be inhibited by an excess of the small regulatory protein, SlrA, and here we find that slrA mRNA accumulated in the pnpA-deletion mutant. Mutation of slrA was epistatic to mutation of pnpA for the motility-related phenotype. Further, Rho-dependent termination was required for PNPase turnover of slrA mRNA. When the slrA gene was provided with a Rho-independent transcription terminator, gene regulation was no longer PNPase-dependent. Thus we show that the slrA transcript is a direct target of PNPase and that regulation of RNA turnover is a major determinant of motility gene expression. The interplay of specific transcription termination and mRNA decay mechanisms suggests selection for fine-tuning of gene expression.
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