ArticleScientific reports2016
De novo transcriptome sequencing and gene expression profiling of spinach (Spinacia oleracea L.) leaves under heat stress.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed.
- Transcription Profiling of Potato Leaves in Response to Heat Stress at Single-Cell Resolution.Plant biotechnology journal · 2026Article
- Sustaining Edible Grass (Antioxidants (Basel, Switzerland) · 2025Article
- Integration of crop modeling and sensing into molecular breeding for nutritional quality and stress tolerance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Review
- Genome-Wide Identification and Characterization of the mTERF Gene Family in Spinach and the Role ofPlants (Basel, Switzerland) · 2025Article
- Article
- Heat-shock transcription factor HsfA8a regulates heat stress response in Sorbus pohuashanensis.Planta · 2024Article
- A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves.Plant methods · 2024Article
- Discovering the regulators of heat stress tolerance inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Comparative transcriptome profiling of contrasting finger millet (Eleusine coracana (L.) Gaertn) genotypes under heat stress.Molecular biology reports · 2024Article
- Analysis of Genes Associated with Feeding Preference and Detoxification in Various Developmental Stages ofInsects · 2024Article
- Bioinformatics in Plant Breeding and Research on Disease Resistance.Plants (Basel, Switzerland) · 2022Review
- Genome-wide identification and expression analysis reveals spinach brassinosteroid-signaling kinase (BSK) gene family functions in temperature stress response.BMC genomics · 2022Article
- Unfolding molecular switches in plant heat stress resistance: A comprehensive review.Plant cell reports · 2022Review
- Molecular mechanisms of plant tolerance to heat stress: current landscape and future perspectives.Plant cell reports · 2021Review
- Expression profiling of the Dof gene family under abiotic stresses in spinach.Scientific reports · 2021Article
- Maize metabolome and proteome responses to controlled cold stress partly mimic early-sowing effects in the field and differ from those of Arabidopsis.Plant, cell & environment · 2021Article
- Comparative transcriptome analyses revealed different heat stress responses in pigeonpea (Cajanus cajan) and its crop wild relatives.Plant cell reports · 2021Article
- Differential Morpho-Physiological and Transcriptomic Responses to Heat Stress in Two Blueberry Species.International journal of molecular sciences · 2021Article
- De novo transcriptomic analysis and identification of EST-SSR markers in Stephanandra incisa.Scientific reports · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inBioMed research international · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spinach (Spinacia oleracea) has cold tolerant but heat sensitive characteristics. The spinach variety 'Island,' is suitable for summer periods. There is lack molecular information available for spinach in response to heat stress. In this study, high throughput de novo transcriptome sequencing and gene expression analyses were carried out at different spinach variety 'Island' leaves (grown at 24 °C (control), exposed to 35 °C for 30 min (S1), and 5 h (S2)). A total of 133,200,898 clean reads were assembled into 59,413 unigenes (average size 1259.55 bp). 33,573 unigenes could match to public databases. The DEG of controls vs S1 was 986, the DEG of control vs S2 was 1741 and the DEG of S1 vs S2 was 1587. Gene Ontology (GO) and pathway enrichment analysis indicated that a great deal of heat-responsive genes and other stress-responsive genes were identified in these DEGs, suggesting that the heat stress may have induced an extensive abiotic stress effect. Comparative transcriptome analysis found 896 unique genes in spinach heat response transcript. The expression patterns of 13 selected genes were verified by RT-qPCR (quantitative real-time PCR). Our study found a series of candidate genes and pathways that may be related to heat resistance in spinach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.